Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: sclerotium
 
NCBI PubMed ID: 15476718
Publication DOI: 10.1016/j.carres.2004.08.003
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: lnzhangpublic.wh.hb.cn
Institutions: Department of Chemistry, Wuhan University, Wuhan 430072, China, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

A water-insoluble (1→3)-β-D-glucan isolated from fresh sclerotium of Poria cocos was, respectively, sulfated, carboxymethylated, methylated, hydroxyethylated, and hydroxypropylated, to afford five water-soluble derivatives. Their weight-average molecular masses (Mw) and intrinsic viscosities ([η]) were determined by size-exclusion chromatography combined with laser light scattering (SEC-LLS), LLS, and viscometry in phosphate buffer solution (PBS) at 37 °C. The antitumor activities, against Sarcoma 180 tumor cell (S-180) and gastric carcinoma cell strain (MKN-45 and SGC-7901) of the native β-glucan and the five derivatives, were tested in vitro and in vivo. The Mw values of the five derivatives in PBS were determined to be 3.8 × 104, 18.9 × 104, 16.0 × 104, 76.8 × 104, and 224.3 × 104, respectively. The high Mw values of the hydroxyethylated and hydroxypropylated derivatives in aqueous solution resulted from aggregation, and their true Mw values obtained in dimethyl sulfoxide were 20.1 × 104 and 19.1 × 104. The sulfated and carboxymethylated derivatives having DS of 1.0–1.3 show good water solubility, and exist as relatively expanded chains in aqueous solution. Interestingly, the native β-glucan did not show antitumor activity, whereas the sulfated and carboxymethylated derivatives exhibit significant antitumor activities against S-180 and gastric carcinoma tumor cells. This work showed that good water solubility, relatively high chain stiffness, and moderate molecular mass of the derivatives in aqueous solution contribute beneficial to enhancement of antitumor activity.

polysaccharide, methylation, light scattering, molecular mass, sulfation, carboxymethylation, antitumor activity, Poria cocos sclerotium, hydroxyethylation, hydroxypropylation

Structure type: homopolymer
Location inside paper: abstract, p.2568, PCS3-II
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
 
Methods: 13C NMR, chemical methods, biological assays, light scattering, viscosity measurement, extraction, elemental analysis, SEC
Biological activity: in vitro and in vivo antitumor activity assays and correlation of structure to antitumor activity
Comments, role: A water-insoluble (1-3)-b-D-glucan was, respectively, sulfated, carboxymethylated, methylated, hydroxyethylated, and hydroxypropylated to obtain five water-soluble derivatives.
 
NCBI Taxonomy refs (TaxIDs): 81056
Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:157, CCSD:50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
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