Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperPublication DOI: 10.1016/j.carbpol.2005.01.002Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: corradi
prudente.unesp.br
Institutions: Departamento de Fisica, Faculdade de Ciencias e Tecnologia, Quimica e Biologia, Universidade Estadual Paulista, CEP 19060-900, Presidente Prudente, SP, Brazil, Departamento de Bioquímica e Biotecnologia, Universidade Estadual de Londrina, CCE, Caixa Postal 6001, CEP 86051-990, Londrina, PR, Brazil, Departamento de Química, Faculdade de Filosofia, Ciências e Letras, Universidade de São Paulo, CEP 14040-901, Ribeirão Preto, SP, Brazil
Two botryosphaerans, exopolysaccharides (EPS) secreted by the ascomyceteous fungus Botryosphaeria rhodina, when grown on sucrose and fructose as sole carbon sources, were structurally compared after their isolation from the culture medium. Both EPS were submitted to trypsin digestion, and eluted as a single peak on gel filtration. Total acid hydrolysis yielded only glucose, and data from methylation analysis and Smith degradation indicated that both EPS constituted a main chain of glucopyranosyl β(1→3) linkages substituted at O-6. The products obtained after partial acid hydrolysis demonstrated side chains consisting of glucosyl- and gentiobiosyl- linked β(1→6) residues. C-13-NMR spectroscopy studies showed that all glucosidic linkages were of the β-configuration. The carbon source affected the side chain structures of botryosphaeran but not the main chain makeup. Sucrose produced less branching (21%) than fructose (31%). (c) 2005 Published by Elsevier Ltd.
exopolysaccharides, Botryosphaeria rhodina, beta(1->3;1->6)-D-glucans, carbon source, structural variation
Structure type: homopolymer
Location inside paper: p.15, table 2
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, methylation, partial acid hydrolysis, sugar analysis, acid hydrolysis, GLC, HPAEC, Smith degradation, GPC, enzymatic digestion, HPAEC-PAD, acetylation
Comments, role: backbone motif identical to the product of Smith degradation; Glucan with a highly branched structure, constituted a (1-3)-linked glucosyl backbone substituted at C-6.
Related record ID(s): 41259
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO at 303(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.1 73.0 86.6 68.7 76.7 61.1
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.1 | 73.0 | 86.6 | 68.7 | 76.7 | 61.1 |
|
There is only one chemically distinct structure:
Found 
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(later renamed to: Lasiodiplodia theobromae)