Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperJournal NLM ID: 101630927WWW link: http://www.researchgate.net/profile/Ting_Jang_Lu/publication/228982907_Molecular_characterization_of_polysaccharides_in_hot-water_extracts_of_Ganoderma_lucidum_fruiting_bodies/links/0912f50e6af3939f67000000.pdfPublisher: Taipei City, Taiwan, R.O.C.: Elsevier
Correspondence: tjlu
ccms.ntu.edu.tw
Institutions: Graduate Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan (R.O.C.)
Polysaccharide components in hot-water extract of Ganoderma lucidum fruiting bodies were separated and their molecular weights and distribution profiles were measured using high-performance size-exclusion chromatography with multi-angle laser light scattering detection (HPSEC-MALLS). Three polysaccharide groups were found in the extract with a weight percentage of 31, 46 and 23%, respectively. The weight-average molecular weights of these groups were 2.08 x 10(6), 2.3 x 10(4) and 1.2 x 10(4) g/mol, respectively. The representative fractions of these 3 groups were obtained using differential precipitation with ethanol at 20, 60 and 80% (v/v) concentrations for further property study. The first group that had the largest molecular size was branched (1→3)-β-D-glucan with single β-D-glucosyl side chains at the O-6 positions, as revealed from sugar composition analysis, aniline blue assay and H1- and C13-NMR spectroscopy. (1→3, 1→6)-β-D-glucan was the major active polysaccharide and showed significant tumor necrosis factor alpha (TNF-α) releasing stimulation activity from human mononuclear cells (MNC). The glucan was slightly soluble in water at ambient temperature. The second and third groups that showed slight modulating activity consisted of D-glucose, D-galactose and D-mannose at different ratios. Aggregation of polysaccharide molecules was also revealed by HPSEC-MALLS study.
polysaccharides, size-exclusion chromatography, Ganoderma lucidum, (1-3, 1-6)-beta-D-glucans, multi-angle laser light scattering, immuno-modulating activity
Structure type: fragment of a bigger structure
Location inside paper: abstract, p.63, table 2, BC (backbone)
Trivial name: glucan particle, PFLP
Compound class: cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, sugar analysis, ELISA, acid hydrolysis, HPSEC-MALLS, extraction, aniline blue assay
Biological activity: (1->3,1->6)-b-D-glucan showed significant TNF-alpha releasing stimulation activity from human mononuclear cells.
Comments, role: Part of structure of branched (1-3,1-6)-b-D-glucan; D-glucan backbone (BC) (see ID: 41330,41331).
Related record ID(s): 41330, 41331
NCBI Taxonomy refs (TaxIDs): 5315Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
NMR conditions: in vol 86%DMSO-d6 / vol 14%D2O at 353(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.1 73.2 86.1-86.7 68.7 76.5 61.2
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.1 | 73.2 | 86.1
86.7 | 68.7 | 76.5 | 61.2 |
|
There is only one chemically distinct structure:
Found 
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