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Alquini G, Carbonero ER, Rosado FR, Cosentino C, Iacomini M
Polysaccharides from the fruit bodies of the basidiomycete Laetiporus sulphureus (Bull.: Fr.) Murr
FEMS Microbiology Letters 230(1) (2004)
47-52
Laetiporus sulphureus
(NCBI TaxID 5630,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Host organism: (plant)
Organ / tissue: fruiting bodyAssociated disease: heart-rot disease
The structure was elucidated in this paperPublication DOI: 10.1016/S0378-1097(03)00853-XJournal NLM ID: 7705721Publisher: Blackwell Publishing
Correspondence: iacomini

bio.ufpr.br
Institutions: Departamento de Bioquímica, Universidade Federal do Paraná, C.P. 19046, 81531-990 Curitiba-PR, Brazil, Departamento de Bioquímica, Universidade Estadual de Maringá, Av. Colombo 5790, 87020-900 Maringá-PR, Brazi, Istituto di Ricerche Chimiche e Biochimiche ‘G. Ronzoni’, Via Colombo 81, 20133 Milan, Italy
The two main polysaccharides from the basidiomycetous fungus Laetiporus sulphureus were isolated, purified and characterized. The structural assignments were carried out using C-13, H-1, and 1H,(13) HSQC nuclear magnetic resonance spectroscopy, methylation analysis, and Smith degradation. One was a linear β-glucan having a (1→3)-linked main chain, namely laminaran. The other was a fucomannogalactan, which consisted of a main chain of (1→6)-linked α-D-galactopyranosyl residues, a part of them being substituted at O-2 by 3-O-D-mannopyranbsyl-L-fucopyranosyl, α-D-mannopyranosyl and in a minor proportion, α-L-fucopyranosyl groups. This heteropolysaccharide is related to those of other Basidiomycetes heterogalactans, although it differs distinctly in its side-chain structures. Whereas part of the single-unit L-fucopyranosyl and/or 3-O-α-mannopyranosyl-L-fucopyranosyl residues are present as side chains of the other heterogalactans, additional a-D-mannopyranosyl units are present in our fucomannogalactan of L. sulphureus.
beta-glucan, heterogalactan, fucomannogalactan, Laetiporus sulphureus
Structure type: homopolymer
Location inside paper: abstract, p.49
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, GC-MS, sugar analysis, acid hydrolysis, Smith degradation, acetylation, reduction, HPSEC-MALLS/RI
Comments, role: The water-insoluble polysaccharide consists of a (1-3)-linked b-glucan, namely laminaran. Infection in deciduous trees and conifers.
Related record ID(s): 41345, 41346, 41347
NCBI Taxonomy refs (TaxIDs): 5630Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
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There is only one chemically distinct structure:
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Alquini G, Carbonero ER, Rosado FR, Cosentino C, Iacomini M
Polysaccharides from the fruit bodies of the basidiomycete Laetiporus sulphureus (Bull.: Fr.) Murr
FEMS Microbiology Letters 230(1) (2004)
47-52
Laetiporus sulphureus
(NCBI TaxID 5630,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Host organism: (plant)
Organ / tissue: fruiting bodyAssociated disease: heart-rot disease
The structure was elucidated in this paperPublication DOI: 10.1016/S0378-1097(03)00853-XJournal NLM ID: 7705721Publisher: Blackwell Publishing
Correspondence: iacomini

bio.ufpr.br
Institutions: Departamento de Bioquímica, Universidade Federal do Paraná, C.P. 19046, 81531-990 Curitiba-PR, Brazil, Departamento de Bioquímica, Universidade Estadual de Maringá, Av. Colombo 5790, 87020-900 Maringá-PR, Brazi, Istituto di Ricerche Chimiche e Biochimiche ‘G. Ronzoni’, Via Colombo 81, 20133 Milan, Italy
The two main polysaccharides from the basidiomycetous fungus Laetiporus sulphureus were isolated, purified and characterized. The structural assignments were carried out using C-13, H-1, and 1H,(13) HSQC nuclear magnetic resonance spectroscopy, methylation analysis, and Smith degradation. One was a linear β-glucan having a (1→3)-linked main chain, namely laminaran. The other was a fucomannogalactan, which consisted of a main chain of (1→6)-linked α-D-galactopyranosyl residues, a part of them being substituted at O-2 by 3-O-D-mannopyranbsyl-L-fucopyranosyl, α-D-mannopyranosyl and in a minor proportion, α-L-fucopyranosyl groups. This heteropolysaccharide is related to those of other Basidiomycetes heterogalactans, although it differs distinctly in its side-chain structures. Whereas part of the single-unit L-fucopyranosyl and/or 3-O-α-mannopyranosyl-L-fucopyranosyl residues are present as side chains of the other heterogalactans, additional a-D-mannopyranosyl units are present in our fucomannogalactan of L. sulphureus.
beta-glucan, heterogalactan, fucomannogalactan, Laetiporus sulphureus
Structure type: fragment of a bigger structure
Location inside paper: p.50, fig.3A
Trivial name: heterogalactan (fucomannogalactan)
Compound class: mannofucogalactan
Contained glycoepitopes: IEDB_130701,IEDB_134624,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_152214,IEDB_153553,IEDB_174333,IEDB_190606,IEDB_983930,SB_154,SB_163,SB_44,SB_67,SB_7,SB_72,SB_86
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, GC-MS, sugar analysis, acid hydrolysis, Smith degradation, acetylation, reduction, HPSEC-MALLS/RI
Comments, role: Error in figure: should be L-Fuc instead of D-Fuc. 18% of backbone fucomannogalactan in the full structure correspond to this fragment. Infection in deciduous trees and conifers.
Related record ID(s): 41344, 41346, 41347
NCBI Taxonomy refs (TaxIDs): 5630Reference(s) to other database(s): GTC:G35659SI
Show glycosyltransferases
1H NMR data: present in publication
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
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Alquini G, Carbonero ER, Rosado FR, Cosentino C, Iacomini M
Polysaccharides from the fruit bodies of the basidiomycete Laetiporus sulphureus (Bull.: Fr.) Murr
FEMS Microbiology Letters 230(1) (2004)
47-52
Laetiporus sulphureus
(NCBI TaxID 5630,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Host organism: (plant)
Organ / tissue: fruiting bodyAssociated disease: heart-rot disease
The structure was elucidated in this paperPublication DOI: 10.1016/S0378-1097(03)00853-XJournal NLM ID: 7705721Publisher: Blackwell Publishing
Correspondence: iacomini

bio.ufpr.br
Institutions: Departamento de Bioquímica, Universidade Federal do Paraná, C.P. 19046, 81531-990 Curitiba-PR, Brazil, Departamento de Bioquímica, Universidade Estadual de Maringá, Av. Colombo 5790, 87020-900 Maringá-PR, Brazi, Istituto di Ricerche Chimiche e Biochimiche ‘G. Ronzoni’, Via Colombo 81, 20133 Milan, Italy
The two main polysaccharides from the basidiomycetous fungus Laetiporus sulphureus were isolated, purified and characterized. The structural assignments were carried out using C-13, H-1, and 1H,(13) HSQC nuclear magnetic resonance spectroscopy, methylation analysis, and Smith degradation. One was a linear β-glucan having a (1→3)-linked main chain, namely laminaran. The other was a fucomannogalactan, which consisted of a main chain of (1→6)-linked α-D-galactopyranosyl residues, a part of them being substituted at O-2 by 3-O-D-mannopyranbsyl-L-fucopyranosyl, α-D-mannopyranosyl and in a minor proportion, α-L-fucopyranosyl groups. This heteropolysaccharide is related to those of other Basidiomycetes heterogalactans, although it differs distinctly in its side-chain structures. Whereas part of the single-unit L-fucopyranosyl and/or 3-O-α-mannopyranosyl-L-fucopyranosyl residues are present as side chains of the other heterogalactans, additional a-D-mannopyranosyl units are present in our fucomannogalactan of L. sulphureus.
beta-glucan, heterogalactan, fucomannogalactan, Laetiporus sulphureus
Structure type: fragment of a bigger structure
Location inside paper: p.50, fig.3B
Trivial name: heterogalactan (fucomannogalactan)
Compound class: mannofucogalactan
Contained glycoepitopes: IEDB_130701,IEDB_134624,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_983930,SB_163,SB_44,SB_67,SB_7,SB_72
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, GC-MS, sugar analysis, acid hydrolysis, Smith degradation, acetylation, reduction, HPSEC-MALLS/RI
Comments, role: 10% of backbone fucomannogalactan in the full structure correspond to this fragment. Infection in deciduous trees and conifers.
Related record ID(s): 41344, 41345, 41347, 48810
NCBI Taxonomy refs (TaxIDs): 5630Reference(s) to other database(s): GTC:G59947NM
Show glycosyltransferases
1H NMR data: present in publication
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Expand this record
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Alquini G, Carbonero ER, Rosado FR, Cosentino C, Iacomini M
Polysaccharides from the fruit bodies of the basidiomycete Laetiporus sulphureus (Bull.: Fr.) Murr
FEMS Microbiology Letters 230(1) (2004)
47-52
Laetiporus sulphureus
(NCBI TaxID 5630,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Host organism: (plant)
Organ / tissue: fruiting bodyAssociated disease: heart-rot disease
The structure was elucidated in this paperPublication DOI: 10.1016/S0378-1097(03)00853-XJournal NLM ID: 7705721Publisher: Blackwell Publishing
Correspondence: iacomini

bio.ufpr.br
Institutions: Departamento de Bioquímica, Universidade Federal do Paraná, C.P. 19046, 81531-990 Curitiba-PR, Brazil, Departamento de Bioquímica, Universidade Estadual de Maringá, Av. Colombo 5790, 87020-900 Maringá-PR, Brazi, Istituto di Ricerche Chimiche e Biochimiche ‘G. Ronzoni’, Via Colombo 81, 20133 Milan, Italy
The two main polysaccharides from the basidiomycetous fungus Laetiporus sulphureus were isolated, purified and characterized. The structural assignments were carried out using C-13, H-1, and 1H,(13) HSQC nuclear magnetic resonance spectroscopy, methylation analysis, and Smith degradation. One was a linear β-glucan having a (1→3)-linked main chain, namely laminaran. The other was a fucomannogalactan, which consisted of a main chain of (1→6)-linked α-D-galactopyranosyl residues, a part of them being substituted at O-2 by 3-O-D-mannopyranbsyl-L-fucopyranosyl, α-D-mannopyranosyl and in a minor proportion, α-L-fucopyranosyl groups. This heteropolysaccharide is related to those of other Basidiomycetes heterogalactans, although it differs distinctly in its side-chain structures. Whereas part of the single-unit L-fucopyranosyl and/or 3-O-α-mannopyranosyl-L-fucopyranosyl residues are present as side chains of the other heterogalactans, additional a-D-mannopyranosyl units are present in our fucomannogalactan of L. sulphureus.
beta-glucan, heterogalactan, fucomannogalactan, Laetiporus sulphureus
Structure type: fragment of a bigger structure
Location inside paper: p.50, fig.3С
Trivial name: heterogalactan (fucomannogalactan)
Compound class: mannofucogalactan
Contained glycoepitopes: IEDB_134624,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142489,IEDB_144562,IEDB_151528,IEDB_152214,IEDB_153553,IEDB_174333,IEDB_190606,SB_154,SB_163,SB_7,SB_86
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, GC-MS, sugar analysis, acid hydrolysis, Smith degradation, acetylation, reduction, HPSEC-MALLS/RI
Comments, role: Minor component of the main chain; error in figure: should be L-Fuc instead of D-Fuc. 2% of backbone fucomannogalactan in the full structure correspond to this fragment. Infection in deciduous trees and conifers.
Related record ID(s): 41344, 41345, 41346
NCBI Taxonomy refs (TaxIDs): 5630Reference(s) to other database(s): GTC:G48279OD
Show glycosyltransferases
1H NMR data: present in publication
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
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