Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: Life stage: mycelial phaseAssociated disease: paracoccidioidomycosis (PCM) [ICD11:
1F2E 
, ICD11:
XN5UX ];
infection due to Paracoccidioides brasiliensis [ICD11:
XN5UX ]
The structure was elucidated in this paperPublication DOI: 10.1093/glycob/cwg069Journal NLM ID: 9104124Publisher: IRL Press at Oxford University Press
Correspondence: Manuel Bernabé <mbernabe
iqog.csic.es>
Institutions: Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006-Madrid, Spain, Centro de Microbiología y Biología Celular, IVIC, P.O. Box 21827, Caracas 1020A, Venezuela, Instituto de Química Orgánica, CSIC, Juan de la Cierva 3, 28006-Madrid, Spain
Paracoccidioides brasiliensis is a pathogenic dimorphic fungus causing paracoccidioidomycosis, the most widespread systemic mycosis in Latin America. We have studied the structure of the alkali-extracted water-soluble cell wall polysaccharides (F1SS) from both mycelial and yeast phases of this fungus by using chemical analysis and NMR spectroscopic techniques. The F1SS polysaccharide from the mycelial phase consists of a trisaccharidic repeating unit of, →6)-[α-Galf-(1→6)-α-Manp-(1→2)]-α-Manp-(1→. The F1SS polysaccharide of the yeast phase maintains 10% of the structure of the mycelium phase, but the main structure contain a disaccharide repeating unit of →6)-[α-Manp-(1→2)]-α-Manp-(1→, alternating with a trisaccharide repeating block of →6)-[β-Galf-(1→6)-α-Manp-(1→2)]-α-Manp-(1→.
NMR spectroscopy, cell wall polysaccharides, fungi, Paracoccidioides brasiliensis
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.744, F1SS (from the mycelial phase)
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137472,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_141829,IEDB_141831,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_190606,IEDB_76933,IEDB_857732,IEDB_857735,IEDB_983930,SB_136,SB_191,SB_196,SB_198,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, partial acid hydrolysis, sugar analysis, acid hydrolysis, GLC, alkali extraction
Comments, role: The alkali-extractable water-soluble cell wall polysaccharide F1SS isolated from the mycelial phase of P. brasiliensis IVIC Pb73 (ATCC 32071).
Related record ID(s): 41354, 41355, 42143
NCBI Taxonomy refs (TaxIDs): 121759Reference(s) to other database(s): GTC:G97994FD
Show glycosyltransferases
NMR conditions: in D2O at 313 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,6 aDGalf 101.2 77.4 75.2 82.1 72.8 63.4
2 aDManp 103.0 70.9 71.3 67.3 72.6 67.0
aDManp 99.0 80.0 71.4 67.1 72.2 66.4
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,6 aDGalf 5.04 4.15 4.22 3.84 3.79 3.63-3.72
2 aDManp 5.02 4.08 3.83 3.81 3.91 3.66-4.05
aDManp 5.08 4.01 3.94 3.87 3.80 3.74-4.04
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,6 aDGalf 101.2/5.04 77.4/4.15 75.2/4.22 82.1/3.84 72.8/3.79 63.4/3.63-3.72
2 aDManp 103.0/5.02 70.9/4.08 71.3/3.83 67.3/3.81 72.6/3.91 67.0/3.66-4.05
aDManp 99.0/5.08 80.0/4.01 71.4/3.94 67.1/3.87 72.2/3.80 66.4/3.74-4.04
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,6 | aDGalf | 5.04 | 4.15 | 4.22 | 3.84 | 3.79 | 3.63
3.72 |
| 2 | aDManp | 5.02 | 4.08 | 3.83 | 3.81 | 3.91 | 3.66
4.05 |
| | aDManp | 5.08 | 4.01 | 3.94 | 3.87 | 3.80 | 3.74
4.04 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,6 | aDGalf | 101.2 | 77.4 | 75.2 | 82.1 | 72.8 | 63.4 |
| 2 | aDManp | 103.0 | 70.9 | 71.3 | 67.3 | 72.6 | 67.0 |
| | aDManp | 99.0 | 80.0 | 71.4 | 67.1 | 72.2 | 66.4 |
|
There is only one chemically distinct structure:
Found 
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