Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 12895548Publication DOI: 10.1016/S0031-9422(03)00336-4Journal NLM ID: 0151434WWW link: http://www.sciencedirect.com/science/article/pii/S0031942203003364Publisher: Elsevier
Correspondence: iacomini
bio.ufpr.br
Institutions: Departamento de Bioquı́mica, Universidade Federal do Paraná, CP 19.046, CEP 81.531-990, Curitiba, PR, Brazil, Institute of Plant Physiology, University of Salzburg, Hellbrunner Str. 34, A-5020 Salzbug, Austria
Several structurally different glucans (alpha- and beta-) and galactomannans were characterized as components of four species of the genus Ramalina, namely R. dendriscoides, R. fraxinea, R. gracilis and R. peruviana. Freeze-thawing treatment of hot aqueous extracts furnished as precipitates (PW) linear α-(D)-glucans of the nigeran type, with regularly distributed (1→3)- and (1→4)-linkages in a 1:1 ratio. The supernatants (SW) contained α-(D)-glucans with (1→3)- and (1→4)-linkages in a molar ratio of 3:1. The lichen residues were then extracted with 2% aq. KOH, and the resulting extracts Submitted to the freeze-thawing treatment, giving rise to precipitates (PK2) of a mixture of α-glucan (nigeran) and β-glucan, which were suspended in aqueous 0.5% NaOH at 50 degreesC, dissolving preferentially the β-glucan. These were linear with (1→3)-linkages (laminaran). The mother liquor of the KOH extractions (2% and 10% aq. KOH) was treated with Fehling's solution to give precipitates (galactomannans). The galactomannans are related. having (1→6)-linked α-(D)-mannopyranosyl main chains, substituted at O-4 and in a small proportion at O-2,4 by β-(D)-galactopyranosyl units. Despite the different habitats of these lichenized fungi, all species studied in this investigation have a similar pool of polysaccharides. (C) 2003 Elsevier Ltd. All rights reserved.
polysaccharides, lichenized fungi, galactomannans, Ramalina peruviana, alpha- and beta-glucans, lichens, Ramalina dendriscoides, Ramalina fraxinea, Ramalina gracilis
Structure type: homopolymer
Location inside paper: graphic abstract, p.971, LAM fraction
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, GC-MS, sugar analysis, acid hydrolysis, HPSEC-MALLS, acetylation, reduction, alkaline extraction, Fehling treatment
Comments, role: Alkaline soluble b-D-glucan laminaran (supernatant LAM).
Related record ID(s): 41365, 41366, 41368
NCBI Taxonomy refs (TaxIDs): 157169,
56479,
2036932,
475428Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 323(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.1 73.1 86.3 68.6 76.5 61.0
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.1 | 73.1 | 86.3 | 68.6 | 76.5 | 61.0 |
|
There is only one chemically distinct structure:
Found 
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