Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 36181715Publication DOI: 10.1002/chem.202202616Journal NLM ID: 9513783Publisher: Weinheim: VCH Verlagsgesellschaft/Verlag I
Correspondence: H.A.B. Wosten <h.a.b.wosten
uu.nl>; M. Baldus <m.baldus
uu.nl>
Institutions: NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht (The, Netherlands, Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht (The, Netherlands, Division of Infectious Diseases and Immunology, Department of Biomolecular Health Sciences, Utrecht University, Yalelaan 1, 3584 CL, Utrecht (The, Netherlands
Solid-state NMR (ssNMR) spectroscopy facilitates the non-destructive characterization of structurally heterogeneous biomolecules in their native setting, for example, comprising proteins, lipids and polysaccharides. Here we demonstrate the utility of high and ultra-high field 1 H-detected fast MAS ssNMR spectroscopy, which exhibits increased sensitivity and spectral resolution, to further elucidate the atomic-level composition and structural arrangement of the cell wall of Schizophyllum commune, a mushroom-forming fungus from the Basidiomycota phylum. These advancements allowed us to reveal that Cu(II) ions and the antifungal peptide Cathelicidin-2 mainly bind to cell wall proteins at low concentrations while glucans are targeted at high metal ion concentrations. In addition, our data suggest the presence of polysaccharides containing N-acetyl galactosamine (GalNAc) and proteins, including the hydrophobin proteins SC3, shedding more light on the molecular make-up of cells wall as well as the positioning of the polypeptide layer. Obtaining such information may be of critical relevance for future research into fungi in material science and biomedical contexts.
NMR, NMR spectroscopy, cell wall, peptide, Schizophyllum commune, proton detection
Structure type: homopolymer
Location inside paper: Fig. S5, chitin
Trivial name: chitin
Compound class: O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, chitin
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: 1H ssNMR
Related record ID(s): 41077, 41480, 41482, 41483, 41484
NCBI Taxonomy refs (TaxIDs): 5334Reference(s) to other database(s): GTC:G97099AY, CCSD:
46067, CBank-STR:5851, GenDB:KF905651
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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