Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 38574411Publication DOI: 10.1016/j.carres.2024.109099Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Milhorini SS <shayane.silva
ufpr.br>
Institutions: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil, Faculdades Pequeno Príncipe, Curitiba, Brazil, Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Brazil
Ganoderma lucidum, widely used in traditional medicine, has several biological properties. Polysaccharides, mainly glucans, are known as one of its main bioactive compounds. Consequently, the achievement and chemical investigation of such molecules are of pharmaceutical interest. Herein, we obtained water-insoluble and water-soluble polysaccharides from G. lucidum by alkaline extraction. Fractionation process yielded three fractions (GLC-1, GLC-2, and GLC-3). All samples showed to be composed mainly of glucans. GLC-1 is a linear (1→3)-linked β-glucan; GLC-2 is a mixture of three different linear polysaccharides: (1→3)-β-glucan, (1→3)-α-glucan, and (1→4)-α-mannan; while GLC-3 is a branched β-glucan with a (1→4)-linked main chain, which is branched at O-3 or O-6 by (1→3)- or (1→6)-linked side chains. This research reports the variability of glucans in Ganoderma lucidum fruiting bodies and applicable methodologies to obtain such molecules. These polysaccharides can be further applied in biological studies aiming to investigate how their chemical differences may affect their biological properties
branched, polysaccharides, linear, mannan, Glucans, Ganoderma lucidum
Structure type: homopolymer
Location inside paper: p.5, left column, paragraph 2
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, acid hydrolysis, HPSEC, extraction, acetylation, methylation analysis, dialysis, precipitation, phenol-sulfuric acid assay, Bradford method, DEPT, centrifugation, fractionation, Fehling treatment
Comments, role: review
Related record ID(s): 41631, 41632, 43323, 45734, 47152, 47304, 49279, 49282, 50035, 50085, 50617, 50648, 50654, 50665, 50671, 50862
NCBI Taxonomy refs (TaxIDs): 5315Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 343 K
1H NMR data: present in publication
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13C NMR data: present in publication
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There is only one chemically distinct structure:
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