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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11: XN6P4

]

The structure was elucidated in this paper
NCBI PubMed ID: 10629949
Publication DOI: 10.1016/S0008-6215(99)00212-8
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: malcolm.perry

nrc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ont., Canada K 1A 0R 6

The antigenic O-polysaccharide moiety of the lipopolysaccharide produced by Escherichia coli serotype O65 was investigated by composition, methylation, base hydrolysis, periodate oxidation, mass spectrometric methods, and by 1D and 2D NMR spectroscopy. The O-polysaccharide had [a]D108° (water) and is a high-molecular-weight unbranched linear polymer of repeating pentasaccharide units composed of 1:1:1:1:1 D-galacturonic acid (D-GalA), D-galacturonamide (D-GalANH2), 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), 2-acetamido-2-deoxy-D-galactose (DGalNAc),and 3-acetamido-3,6-dideoxy-D-glucose (D-Qui3NAc), and has the following structure: [see formula in text]

Lipopolysaccharide, Escherichia coli, NMR spectroscopy, polysaccharide structure

Structure type: oligomer
Location inside paper: p.63
Contained glycoepitopes: IEDB_130648,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_151531,IEDB_885822

Methods: methylation, periodate oxidation, NMR-2D, NMR, MS, composition analysis, base hydrolysis

Related record ID(s): 4043
NCBI Taxonomy refs (TaxIDs): 2162912
Reference(s) to other database(s): GlycomeDB:36771
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@@H]([C@H](O)[C@@H](C)O)[C@H](CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@@H]2O[C@H](C(=O)O)[C@H](O[C@H]3O[C@H](CO)[C@H](O[C@H]4OC(C(=O)O)=C[C@H](O)[C@H]4O)[C@H](O)[C@H]3NC(C)=O)[C@H](O)[C@H]2O)[C@H]1NC(C)=O

SVG MW SMILES 3D mol Ionize