Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperPublication DOI: 10.1016/j.carres.2010.02.028Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Syed S. Islam <sirajul_1999
yahoo.com>
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, West Bengal, India, Plant Molecular and Cellular Genetics Section, Bose Institute, Kolkata, West Bengal, India
A water-soluble immunoenhancing polysaccharide was isolated from the aqueous extract of fruit bodies of somatic hybrid (Pflo Vv5 FB), obtained through protoplast fusion between Pleurotus florida and Volvariella volvacea strains. On the basis of acid hydrolysis, the polysaccharide was found to contain glucose only. Methylation analysis, periodate oxidation along with 1H, DEPT-135, and 13C NMR spectroscopy, including two-dimensional TOCSY, DQF-COSY, NOESY, ROESY, 1H,13C-HMQC, and HMBC experiments showed that the polysaccharide was a (1→6)-β-D-glucan, which was not a constituent of any of the parent mushrooms previously reported. This glucan stimulated the macrophages, splenocytes, and thymocytes.
structure, polysaccharide, NMR spectroscopy, Pleurotus florida, Somatic hybrid, Volvariella volvacea
Structure type: homopolymer ; 185000
Location inside paper: abstract, p.976
Trivial name: 1,6-β-glucan
Compound class: EPS, glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, periodate oxidation, GLC-MS, NMR-2D, sugar analysis, acid hydrolysis, biological assays, paper chromatography, genetic methods, GPC, methylation analysis
Biological activity: The glucan was found to activate macrophages and to increase proliferation of splenocytes and thymocytes
Comments, role: EPS was obtained from somatic hybrid (Pflo Vv5 FB) fruiting body, obtained through protoplast fusion between Pleurotus florida and Volvariella volvacea strains.
NCBI Taxonomy refs (TaxIDs): 188765,
36659Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.2 73.5 76.1 70.0 75.3 69.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.53 3.35 3.51 3.47 3.65 3.91-4.23
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 103.2/4.53 73.5/3.35 76.1/3.51 70.0/3.47 75.3/3.65 69.1/3.91-4.23
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.53 | 3.35 | 3.51 | 3.47 | 3.65 | 3.91
4.23 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.2 | 73.5 | 76.1 | 70.0 | 75.3 | 69.1 |
|
There is only one chemically distinct structure:
Found 
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(later renamed to: Pleurotus sp. Florida x Volvariella volvacea)