Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 19853245Publication DOI: 10.1016/j.carres.2009.09.029Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Kruppa
etsu.edu (Michael D. Kruppa)
Institutions: Department of Microbiology, East Tennessee State University, James H Quillen College of Medicine, Johnson City, Tennessee, USA, Department of Surgery, East Tennessee State University, James H. Quillen College of Medicine, Johnson City, Tennessee, USA, University of Guelph, Guelph, Ontario, Canada, Department of Medicine Solna, Karolinska Institutet, Clinical Allergy Research Unit, Stockholm, Sweden
Members of the genus Malassezia are commensal fungi found on the skin of both human and domestic animals and are associated with skin diseases including dandruff/seborrheic dermatitis, pityriasis versicolor, and atopic eczema (AE) in humans. In this study we have characterized the cell-wall carbohydrates of Malassezia sympodialis, one of the species most frequently isolated from both AE patients and healthy individuals. Cells were grown in liquid Dixon media at 32 degrees C, harvested, and processed using a standard Fehling's precipitation methodology for the isolation of mannan and a standard base/acid extraction for (1→3)-β-D-glucans. Using these classic extraction methods we were unable to isolate precipitable mannan or insoluble (1→3)-β-D-glucan. However, acidification and addition of methanol to the remaining Fehling's-treated sample resulted in a very clean precipitate. This material was characterized by GPC-MALLS, 1D and 2D NMR, and GC-MS for monomer-type and linkage-type composition. We determined that trace amounts of both mannan and branched (1→3, 1→6)-β-D-glucan were present in the recovered precipitate, but not linear (1→3)-β-D-glucan. Surprisingly, NMR analysis indicated that (1→6)-β-D-glucan was the major carbohydrate component isolated from M. sympodialis cell wall. GC-MS linkage analysis confirmed the (1→6)-β-D-glucan structure. Based on these studies we have determined that the M. sympodialis cell wall contains (1→6)-β-D-glucan as the major carbohydrate component along with trace amounts of mannan and (1→3, 1→6)-β-d-glucan. In addition, these data indicate that modification of the classic mannan isolation methodology may be useful in the simultaneous isolation of both mannan and (1→6)-β-D-glucan from other fungi.
(1-6)-β-D-Glucan, Malassezia sympodialis cell wall, Carbohydrate extraction
Structure type: homopolymer ; 8500, 60000
Location inside paper: p.2476, fig.2C, table 2
Trivial name: (1-6)-β-D-glucan
Compound class: cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, composition analysis, extraction, reduction with NaBD4, GPC-MALLS, fluorescent staining of cell-wall
NCBI Taxonomy refs (TaxIDs): 1230383Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.1 73.1 75.7 69.6 75.0 68.9
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.55 3.34 3.53 3.50 3.65 3.88-4.23
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 103.1/4.55 73.1/3.34 75.7/3.53 69.6/3.50 75.0/3.65 68.9/3.88-4.23
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.55 | 3.34 | 3.53 | 3.50 | 3.65 | 3.88
4.23 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.1 | 73.1 | 75.7 | 69.6 | 75.0 | 68.9 |
|
There is only one chemically distinct structure:
Found 
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