Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Associated disease: infection due to Cryptococcus neoformans [ICD11:
XN3EH 
]
NCBI PubMed ID: 19285564Publication DOI: 10.1016/j.fgb.2009.03.003Journal NLM ID: 9607601Publisher: Orlando, FL : Academic Press / Elsevier
Correspondence: marciolr
pq.cnpq.br
Institutions: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA, Laboratório de Estudos Integrados em Bioquímica Microbiana, Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Cidade Universitária CCS, Rio de Janeiro - RJ, Brazil, Division of Infectious Diseases of the Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA, Disciplina de Biologia Celular, Universidade Federal de São Paulo, São Paulo, SP, Brazil
The virulence attributes of Trichosporon asahii are virtually unknown, despite its growing relevance as causative agent of superficial and invasive diseases in humans. Glucuronoxylomannan (GXM) is a well described virulence factor of pathogenic species in the Cryptococcus genus. GXM is also produced by species of the Trichosporon genus, and both polysaccharides share antigenic determinants, but unlike cryptococcal GXM, relatively little work has been done on trichosporal GXMs. In this study, we analyzed structural and functional aspects of GXM produced by T. asahii and compared them to the properties of the cryptococcal polysaccharide. Trichosporal and cryptococcal GXM shared antigenic reactivity, but the former polysaccharide had smaller effective diameter and negative charge. GXM anchoring to the cell wall was perturbed by dimethylsulfoxide and required interactions of chitin-derived oligomers with the polysaccharide. GXM from T. asahii supernatants are incorporated by acapsular mutants of Cryptococcus neoformans, which renders these cells more resistant to phagocytosis by mouse macrophages. In summary, our results establish that despite similarities in cell wall anchoring, antigenic and antiphagocytic properties, trichosporal and cryptococcal GXMs manifest major structural differences that may directly affect polysaccharide assembly at the fungal surface.
phagocytosis, Glucuronoxylomannan, Trichosporon
Structure type: structural motif or average structure
Location inside paper: p.497, fig.1A
Trivial name: glucuronoxylomannan (GXM), glucuronoxylomannan (GXM) Motif M2
Compound class: CPS, EPS, cell wall polysaccharide, glucuronoxylomannan, glucuronoxylomannan (GXM)
Contained glycoepitopes: IEDB_114701,IEDB_115136,IEDB_115576,IEDB_130701,IEDB_140116,IEDB_140630,IEDB_144983,IEDB_145668,IEDB_152206,IEDB_164174,IEDB_167188,IEDB_174332,IEDB_2270799,IEDB_423153,IEDB_76933,IEDB_983930,SB_197,SB_44,SB_67,SB_72
Methods: GC-MS, ELISA, composition analysis, serological methods, enzymatic digestion, zeta potential measurement, binding assays, fluorescence microscopy, flow cytometry analysis, phagocytosis assay
Related record ID(s): 41955, 43555
NCBI Taxonomy refs (TaxIDs): 178876Reference(s) to other database(s): GTC:G75580HK
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error
(later renamed to: Cryptococcus neoformans var. grubii)