Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: mycelium
The structure was elucidated in this paperNCBI PubMed ID: 18237722Publication DOI: 10.1016/j.carres.2007.12.021Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: corradi
fct.unesp.br
Institutions: Departamento de Física, Química e Biologia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, Presidente Prudente, São Paulo, Brazil, Universidad de Castilla—La Mancha, Instituto de Regional Investigación Científica Aplicada (IRICA), Real, Spain, Departamento de Bioquímica e Biotecnologia—CCE, Universidade Estadual de Londrina, Londrina, Paraná, Brazil, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil
Three D-glucans were isolated from the mycelium of the fungus Botryosphaeria rhodina MAMB-05 by sequential extraction with hot-water and hot aqueous KOH (2% w/v) followed by ethanol precipitation. Following their purification by gel permeation chromatography on Sepharose CL-4B, the structural characteristics of the D-glucans were determined by FT-IR and 13C NMR spectroscopy and, after methylation, by GC-MS. The hot-water extract produced a fraction designated Q1A that was a β-(1→6)-D-glucan with the following structure: [Formula: see text] The alkaline extract, when subjected to repeated freeze-thawing, yielded two fractions: K1P (insoluble) that comprised a β-(1→3)-D-glucan with β-D-glucose branches at C-6 with the structure: [Formula: see text] and K1SA (soluble) consisting of a backbone chain of α-(1→4)-linked D-glucopyranosyl residues substituted at O-6 with α-D-glucopyranosyl residues: [Formula: see text]
Botryosphaeria rhodina MAMB-05, Fungal cell wall polysaccharides, d-Glucans
Structure type: homopolymer
Location inside paper: abstract, p.794, p.797, table 2, fraction Q1
Trivial name: pustulan, β-1,6-glucan, β-1,6-D-glucan, β(1-6)-D-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-(1,6)-glucan, lasiodiplodan, β-(1,6)-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-1,6-glucan, β-(1,6)-glucan, pustulan, β-(1→6)-glucan PCPS, water-soluble glucan (PS-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glycoprotein, glucan, polysaccharide, cell wall glucoprotein
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, methylation, GC-MS, sugar analysis, acid hydrolysis, FTIR, GPC, HPAEC-PAD, extraction, acetylation, reduction
Comments, role: The hot-water extract produced a fraction designated Q1A.
Related record ID(s): 41969, 41971
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863, CCSD:
50854, CBank-STR:4234
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 303(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 102.96 73.22 75.89 69.93 75.06 69.01
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 102.96 | 73.22 | 75.89 | 69.93 | 75.06 | 69.01 |
|
There is only one chemically distinct structure:
Found 
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(later renamed to: Lasiodiplodia theobromae MAMB-05)