Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 18639868Publication DOI: 10.1016/j.carres.2008.06.013Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: corradi
fct.unesp.br (M. de Lourdes Corradi da Silva)
Institutions: Departamento de Física, Química e Biologia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, Presidente Prudente, São Paulo, Brazil, Departamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual Paulista, Rio Claro, São Paulo, Brazil, Universidad de Castilla—La Mancha, Instituto de Regional Investigación Científica Aplicada (IRICA), Real, Spain, Departamento de Bioquímica e Biotecnologia—CCE, Universidade Estadual de Londrina, Londrina, Paraná, Brazil, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil, Departamento de Química e Ciências Ambientais, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, São Paulo, Brazil
Four exopolysaccharides (EPS) obtained from Botryosphaeria rhodina strains isolated from rotting tropical fruit (graviola, mango, pinha, and orange) grown on sucrose were purified on Sepharose CL-4B. Total acid hydrolysis of each EPS yielded only glucose. Data from methylation analysis and (13)C NMR spectroscopy indicated that the EPS from the graviola isolate consisted of a main chain of glucopyranosyl (1→3) linkages substituted at O-6 as shown in the putative structure below: [carbohydrate structure: see text]. The EPS of the other fungal isolates consisted of a linear chain of (1→6)-linked glucopyranosyl residues of the following structure: [carbohydrate structure: see text]. FTIR spectra showed one band at 891 cm(-1), and (13)C NMR spectroscopy showed that all glucosidic linkages were of the β-configuration. Dye-inclusion studies with Congo Red indicated that each EPS existed in a triple-helix conformational state. β-(1→6)-d-Glucans produced as exocellular polysaccharides by fungi are uncommon.
exopolysaccharides, Botryosphaeria rhodina isolates, β(1-6)-d-Glucans, β(1-3;1-6)-d-Glucans, Triple-helix conformation
Structure type: homopolymer
Location inside paper: abstract, p.2482, fig.1, glucan (2), EPSSUC-2, EPSSUC-3, EPSSUC-4
Trivial name: pustulan, β-1,6-glucan, β-1,6-D-glucan, β(1-6)-D-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-(1,6)-glucan, lasiodiplodan, β-(1,6)-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-1,6-glucan, β-(1,6)-glucan, pustulan, β-(1→6)-glucan PCPS, water-soluble glucan (PS-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glycoprotein, glucan, polysaccharide, cell wall glucoprotein
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, methylation, GC-MS, sugar analysis, conformation analysis, acid hydrolysis, FTIR, GPC, HPAEC-PAD, dialysis
Comments, role: B. rhodina MMMFR (mango, Mangifera sp.), B. rhodina MMPI (pinha, Annona squamosa), and B. rhodina MMLR (orange, Citrus sp.).
Related record ID(s): 41760
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863, CCSD:
50854, CBank-STR:4234
Show glycosyltransferases
[as TSV]
13C NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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(later renamed to: Lasiodiplodia theobromae MMMFR)