Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperPublication DOI: 10.1016/j.carbpol.2006.03.009Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Iacomini M <iacomini
ufpr.br>
Institutions: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brazil
The glucans of basidiomycetes are an important class of polysaccharides with potential biological activities. In this work, the β-glucans were isolated from the fruiting bodies of edible mushrooms, Pleurotus eryngii and Pleurotus ostreatoroseus, via extraction with hot water, and then fractionation by freeze-thawing. The insoluble glucans gave similar 13C NMR spectra, monosaccharide composition and methylation analyses, and P. eryngii was selected for further controlled Smith degradation, and DEPT and 1H (obs.), 13C HMQC spectroscopy. It was a branched β-glucan, with a main chain of (1→3)-linked-Glcp residues, substituted at O-6 by single-unit β-Glcp side-chains, on average to every third residue of the backbone, as in scleroglucan.
polysaccharides, β-Glucans, Edible mushrooms, Pleurotus spp.
Structure type: homopolymer
Location inside paper: p. 255, SM-PE
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GC-MS, acid hydrolysis, Smith degradation, composition analysis, extraction, permethylation, reduction with NaBD4, acetylation, dialysis, DEPT
Comments, role: Smith-degraded insoluble glucan (IEPW-PE).
Related record ID(s): 41780
NCBI Taxonomy refs (TaxIDs): 5323Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 343(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 102.9 72.8 86.1 68.4 76.3 60.9
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 102.9 | 72.8 | 86.1 | 68.4 | 76.3 | 60.9 |
|
There is only one chemically distinct structure:
Found 
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