Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: lichen thallus
The structure was elucidated in this paperPublication DOI: 10.1639/0007-2745(2005)108[118:PPBTCP]2.0.CO;2Journal NLM ID: 100955480Publisher: American Bryological and Lichenological Society
Correspondence: lucimaramcc
yahoo.com, iacomini
ufpr.br
Institutions: Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná—UNIOESTE, CEP 85819-110, Cascavel, PR, Brazil, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-990, Curitiba, PR, Brazil
Polysaccharides of foliose thalli of Pseudocyphellaria clathrata (De Not.) Malme, a fungus lichenized with a green photobiont, were extracted and chemically characterized. The thalli were successively extracted with water and 2% aqueous KOH, at 100 degrees C. After neutralization, the solution was subjected to freeze-thawing and Fehling treatment of the supernatant precipitated a structurally complex galactoglucomannan (GGM) that was identical to a similar fraction obtained from Pseudocyphellaria aurata (Ach.) Vainio, a fungus lichenized by the green alga Dictyochloropsis symbiotica. Isolated from the supernatant of the Fehling precipitation, was an arabinogalactomannoglucan (AGMG) that contained arabinose, galactose, mannose, and glucose in a 12:34:40:14 molar ratio. Methylation analysis showed 18 different structures, the principal being nonreducing galactofuranosyl end-units (25%) with the main chain composed by (1→3)-linked β-glucopyranosyl units, as seen by controlled Smith degradation.
polysaccharides, lichen, Pseudocyphellaria clathrata
Structure type: structural motif or average structure
Location inside paper: p.120
Trivial name: β-1,3-glucan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, GC-MS, acid hydrolysis, Smith degradation, composition analysis, HPSEC, acetylation, alkaline extraction
Comments, role: The backbone structure of arabinogalactomannoglucan (AGMG) obtained via a controlled Smith degradation.
Related record ID(s): 41277, 41278
NCBI Taxonomy refs (TaxIDs): 534608Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error