Taxonomic group: fungi /
NCBI PubMed ID: 2207281Publication DOI: 110.1002/bip.360291402Journal NLM ID: 0372525Publisher: Wiley Interscience
Institutions: Biophysics Division, National Cancer Center Research Institute, Tsukiji 5-Chorne, Chuo-ku, Tokyo 104, Japan, Chemotherapy Division, National Cancer Center Research Institute, Tsukiji 5-chorne, Chuo-ku, Tokyo 104, Japan
We have recorded high-resolution 13C-NMR spectra of linear (curdlan) and branched (lentinan, HA-β-glucan and its polyol and aldehyde derivatives) (1-3)-β-D-glucans in hydrate and gel states, in order to gain insight into their gelation mechanism. Network structure of curdlan turned out to be highly heterogeneous from its motional state, from liquid-like, through intermediate, to solid-like domains. They are studied by a variety of experiments, conventional high-resolution NMR by broad-band decoupling, high-power decoupling with magic angle spinning (MAS), and cross-polarization-magic-angle-spinning (CP-MAS). Nevertheless, we found that conformations of these distinct liquid-like and solid-like do- mains exhibit an identical single helix conformation with a small proportion of a triple helix form, supporting our previous view as to the gelation mechanism. In contrast, the network structure of branched (1-3)-β-D-glucans in the gel state arises mainly from the triple helix conformation. This means that gelation of branched (1-3)-β-D-glucan proceeds from partial association of the triple helical chains, previously proposed for gelation of a linear glucan. Furthermore, we found that conversion from the single chain to the single helix was not achieved readily by hydration of over 8h at 96% R. H. for branched glucan but the triple helix form is obtained when these samples are hydrated fully as in gel state.
conformation, 13C NMR, Curdlan, lentinan
Structure type: homopolymer
Location inside paper: p.1690, scheme 1, curdlan, I
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, periodate oxidation, X-ray, conformational analysis
Comments, role: The NMR temperature was not indicated; C13-NMR spectra of curdlan in hydrate state.
Related record ID(s): 42117, 42118, 42119
NCBI Taxonomy refs (TaxIDs): 4751Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 104.3 73.8 87.3 69.1 75.8 61.2
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 104.3 | 73.8 | 87.3 | 69.1 | 75.8 | 61.2 |
|
There is only one chemically distinct structure:
report error
Found 1 record.
Displayed record 1
