Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 26048011Publication DOI: 10.1128/EC.00032-154Journal NLM ID: 101130731Publisher: American Society for Microbiology
Correspondence: free
buffalo.edu
Institutions: Department of Biological Sciences, SUNY University at Buffalo, Buffalo, NY, USA, School of Dental Medicine, Periodontics and Endodontics, SUNY University at Buffalo Dental School, Buffalo, NY, USA
A biochemical pathway for the incorporation of cell wall protein into the cell wall of Neurospora crassa was recently proposed. In this pathway, the DFG-5 and DCW-1 endo-α-1,6-mannanases function to covalently cross-link cell wall protein-associated N-linked galactomannans, which are structurally related to the yeast outer chain mannans, into the cell wall glucan-chitin matrix. In this report, we demonstrate that the mannosyltransferase enzyme Och1p, which is needed for the synthesis of the N-linked outer chain mannan, is essential for the incorporation of cell wall glycoproteins into the Candida albicans cell wall. Using endoglycosidases, we show that C. albicans cell wall proteins are cross-linked into the cell wall via their N-linked outer chain mannans. We further demonstrate that the Dfg5p and Dcw1p α-1,6-mannanases are needed for the incorporation of cell wall glycoproteins into the C. albicans cell wall. Our results support the hypothesis that the Dfg5p and Dcw1p α-1,6-mannanases incorporate cell wall glycoproteins into the C. albicans cell wall by cross-linking outer chain mannans into the cell wall glucan-chitin matrix.
cell wall, mannan, N-glycans, endo-mannanases
Structure type: structural motif or average structure
Location inside paper: p. 792, column 1, paragraph 1
Trivial name: chitin
Compound class: cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Biological activity: cross-link the oligosaccharides associated with the GPI anchor into the cell wall glucan-chitin matrix
Related record ID(s): 42315, 42316
NCBI Taxonomy refs (TaxIDs): 5476,
4932Reference(s) to other database(s): GTC:G97099AY
Show glycosyltransferases
There is only one chemically distinct structure:
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