Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 25839826Publication DOI: 10.1016/j.carbpol.2015.02.031Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: ecoelho
ua.pt
Institutions: QOPNA Departamento de Química, Universidade de Aveiro, Aveiro, Portugal
The cell wall polysaccharides of brewers spent yeast Saccharomyces pastorianus (BSY) and the inoculum yeast (IY) were studied in order to understand the changes induced by the brewing process. The hot water and alkali extractions performed solubilized mainly mannoproteins, more branched for BSY than those of IY. Also, (31)P solid state NMR showed that the BSY mannoproteins were 3 times more phosphorylated. By electron microscopy it was observed that the final residues of alkali sequential extraction until 4M KOH preserved the yeast three-dimensional structure. The final residues, composed mainly by glucans (92%), showed that the BSY, when compared with IY, contained higher amount of (1→4)-linked Glc (43% for BSY and 16% for IY) and lower (1→3)-linked Glc (17% for BSY and 42% for IY). The enzymatic treatment of final residue showed that both BSY and IY had (α1→4)-linked Glc and (β1→4)-linked Glc, in a 2:1 ratio, showing that S. pastorianus increases their cellulose-like linkages with the brewing process.
glucan, mannoprotein, alcoholic fermentation stress, brewers spent yeast, sequential extraction
Structure type: structural motif or average structure
Location inside paper: p. 328, column 2, paragraph 3
Trivial name: β-1,3-glucan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: extraction, HR-MAS NMR, dialysis, enzymatic treatments, GC-FID, precipitation, SEM, sonication, centrifugation, GC-qMS
Related record ID(s): 42322, 42323, 42325
NCBI Taxonomy refs (TaxIDs): 27292Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
There is only one chemically distinct structure:
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