Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 25631683Publication DOI: 10.1016/j.imbio.2015.01.002Journal NLM ID: 8002742Publisher: Amsterdam: Elsevier
Correspondence: uholmskov
health.sdu.dk
Institutions: Institute of Molecular Medicine, Department of Cancer and Inflammation, University of Southern Denmark, Odense, Denmark
Aspergillus fumigatus (A. fumigatus) is a ubiquitous fungus that activates, suppresses or modulates the immune response by changing its cell wall structure and by secreting proteases. In this study, we show that chitin acts as an adjuvant in a murine model of A. fumigatus protease induced allergy. The mice were immunised intraperitoneally with A. fumigatus culture filtrate antigen either with or without chitin and were subsequently challenged with the culture filtrate antigen intranasally. Alum was used as an adjuvant control. Compared to alum, chitin induced a weaker inflammatory response in the lungs, measured as the total cell efflux in BAL, EPO and chitinase production. However, chitin enhanced the total IgE, specific IgE and specific IgG1 production as efficiently as alum. Pre-treatment with chitin but not with alum depressed the concentration of the Th2 cytokines IL-4 and IL-13 in BAL fluid. These results shows that chitin, in spite of a reduction of the Th2 cytokine levels in the lungs, enhanced the total and specific IgE production in A. fumigatus culture filtrate induced allergy.
innate immunity, IgE, Aspergillus fumigatus, chitin, Fungal cell wall polysaccharides, lung inflammation
Structure type: structural motif or average structure
Location inside paper: p. 714, columnn 1, paragraph 1
Trivial name: chitin
Compound class: cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: biological assays
Biological activity: directs the immune responses; mediating inhibition of T-cell proliferation; inducing innate pro-inflammatory Th2 directed immune responses; dampening Th1/Th17 induced gut inflammation; adjuvant in an allergy model using 1WCF; increasing the Th2 directed specific immune response against the allergen; enhancing the Th1 cytokines in the lungs
Related record ID(s): 101632, 111874, 121703, 131817, 139821, 139869, 143656, 143703, 145244, 147175, 147987, 149428, 149871, 149874, 149876
NCBI Taxonomy refs (TaxIDs): 746128Reference(s) to other database(s): GTC:G97099AY
Show glycosyltransferases
There is only one chemically distinct structure:
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