Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperPublication DOI: 10.1016/j.carbpol.2009.10.023Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Liu J <jichengliu
yahoo.cn>; Liu J <qyybliu
126.com>
Institutions: Department of Medicine Research, Qiqihar Medical University, Qiqihar, China
In this research, one low molecular weight polysaccharide (LMW-ABP) was isolated from the fruiting body of Agaricus blazei Murill. We reported that the effect of LMW-ABP on inhibiting the interaction between E-selectin and sialyl Lewis X (sLex) by flow cytometry and real-time reverse transcription polymerase chain reaction (RT-PCR) technology. Different concentrations of LMW-ABP (5, 10, and 20 mg/L) could effectively dose-dependently inhibit adhesion of HT-29 cells to human umbilical vein endothelial cells (HUVECs) in static conditions, as well as down-regulating the expression of both α-1,3-fucosyltransferase-VII (FucT-VII) and sLex in transcription or translation level, respectively. These results suggest that LMW-ABP could suppress the metastasizing capacity of cancer cells through interfering with the interaction between E-selectin and sLex. Thus based on these findings, LMW-ABP is expected to be having enormous potential for use in treatment for neoplasm metastasis.
polysaccharide, adhesion, α-1, Agaricus blazei, E-selectin, Sialyl Lewis X, 3-fucosyltransferase-VII
Structure type: polymer chemical repeating unit ; 48000
Location inside paper: p.922, column 1, paragraph 1, LMW-ABP
Compound class: glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: biological assays, RT-PCR, DEAE, fluorescence microscopy, dialysis, flow cytometry analysis, centrifugation, phenol/sulphuric acid method of Dubois, Lowry method, ANOVA
Biological activity: supresses the expression of sLex and FucT-VII mRNA on the surface of HT-29 cells; can be potential cnadidate for developing a novel low toxicity therapeutical agent for neoplasm metastasis
NCBI Taxonomy refs (TaxIDs): 79798Reference(s) to other database(s): GTC:G51056AN
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There is only one chemically distinct structure:
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