Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 25316421Publication DOI: 10.1016/j.ijbiomac.2014.09.053Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Deng C <jscdcdjl
163.com>, Cheng Y <chengym2008
126.com>
Institutions: Wuxi Medical School, Jiangnan University, Wuxi, China, School of Pharmaceutics Science, Jiangnan University, Wuxi, China, Wuxi No. 4 people’s Hospital, Wuxi, China
In this study, we aim to investigate the physiochemical and biological properties of water-soluble phosphorylated polysaccharides (P-DIP) obtained from a water-insoluble polysaccharide (DIP) extracted from Dictyophora indusiata. A series of physiochemical properties were determined, including morphology, water-solubility, molecular weight, and degree of substitution (DS). To investigate the antioxidant activity of P-DIP, we determined the scavenging activity of hydroxyl radicals and DPPH, as well as the reducing power. MTT assay was performed to determine the cytotoxic effects of DIP and P-DIP on the cellular proliferation of MCF-7 and B16 cells. Compared with DIP, P-DIP showed a satisfactory water-solubility and significant increase in the antioxidant properties. Moreover, P-DIP also showed more significant inhibitory effects on the growth of MCF-7 and B16 tumor cells than the water-insoluble DIP. These results indicated that phosphorylation might contribute to the improvement of water solubility, as well as antioxidant and anti-tumor activities of natural DIP.
phosphorylation, Dictyophora indusiata, anti-oxidant and anti-tumor activity, dialyisis, centrifugation
Structure type: homopolymer
Location inside paper: p. 894, column 2, paragraph 1
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, IR, GC-MS, GPC, phosphorylation, solubility assays, antioxidant activities, phenol-sulfuric acid assay, antitumor activity assay, MTT, carbazole method, ANOVA, radical scavenging assays
Related record ID(s): 42466, 42468
NCBI Taxonomy refs (TaxIDs): 81056Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
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There is only one chemically distinct structure:
Found 
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(later renamed to: Wolfiporia cocos)