Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: sclerotium
Publication DOI: 10.1016/S0008-6215(98)00105-0Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Wu C <chiwu
cuhk.edu.hk>
Institutions: Department of Chemistry, Wuhan University, Wuhan, China, Department of Chemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China, The Open Laboratory of Bond-Selective Chemistry, Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui, China
Three fractions of a pachyman sample extracted from poria cocos sclerotium in both Me2SO and a 0.5 N NaOH aqueous solution were investigated by static and dynamic laser light scattering (LLS). In static LLS, the angular dependence of the excess time-average scattering intensity led to the weight-average molecular weight Mw, while in dynamic LLS, the Laplace inversion of a precisely measured intensity-intensity correlation function resulted in a translational diffusion coefficient distribution G(D). A combination of static and dynamic LLS results enabled us to establish a calibration of D (cm2/s)=3.6×10^−4 M−0.674. Using this calibration, we were able to convert each G(D) into a corresponding molar mass distribution F(M). We found that the ratio of the radius of gyration to the hydrodynamic radius was greater than 2, indicating that the pachyman chains have an extended conformation in Me2SO. Our results also revealed the formation of large pachyman aggregates with a loose structure in aqueous NaOH solution.
polysaccharide, light scattering, aggregation, Fractionation, pachyman
Structure type: homopolymer ; 457000 (FR-1), 111000 (FR-2), 238000 (FR-3)
Location inside paper: FR-1, FR-2, FR-3, pachyman, in text, Table 2
Trivial name: pachyman
Compound class: glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: dynamic light scattering
NCBI Taxonomy refs (TaxIDs): 81056Reference(s) to other database(s): GTC:G51056AN
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There is only one chemically distinct structure:
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(later renamed to: Wolfiporia cocos)