Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: sclerotium
The structure was elucidated in this paperPublication DOI: 10.1016/S0021-9673(99)00086-2Journal NLM ID: 9318488Publisher: Amsterdam; New York: Elsevier
Correspondence: Zhang L <lnzhang
elm.pdexpress.com>
Institutions: Department of Chemistry, Wuhan University, Wuhan, China, Agricultural Testing Center of Hubei Academy of Agriculture Science, Wuhan, China
β-D-Glucan PC3 (weight-average molecular mass=9.15*10^4, polydispersity=1.6), a linear glucan from Poria cocos sclerotium, was fractionated by a preparative size-exclusion chromatography (SEC) column packed with regenerated cellulose gels using dimethyl sulfoxide (DMSO) as mobile phase. The molecular masses and aggregation of the fractions were investigated by using analytical SEC, viscometry and membrane osmometry under different conditions. The results of SEC analysis proved that glucan PC3 forms partial aggregates in 0.25 M LiCl/DMSO (namely, 0.25 M LiCl in DMSO) at 40°C, showing a new shoulder peak of a higher-molecular-mass component in the chromatogram, and dissolves as a single-stranded chain at 80°C, giving a sharper peak. The mean apparent aggregation number (Nap) of the fractions in 0.25 M LiCl/DMSO at 40°C was 1.9, and much smaller than that of glucan PC3 in 20% cadoxen. It is indicated that the analytical SEC is more sensitive and direct in detection of the aggregates than other methods, and aggregation can be avoided by carrying out SEC measurement at 80°C. The Mark-Houwink equation for glucan PC3 in DMSO at 25°C was established as [η]=0.107Mw0.50 in the Mw range of 7-11*10^4, suggesting that it behaves as a flexible coil.
carbohydrates, Poria cocos, preparative chromatography
Structure type: homopolymer ; 91500
Location inside paper: PC3, β-D-Glucan, in text
Compound class: glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: HPLC, viscosity measurement, SEC
NCBI Taxonomy refs (TaxIDs): 81056Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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(later renamed to: Wolfiporia cocos)