Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 9043939Publication DOI: 10.1016/s0162-3109(96)00157-9Journal NLM ID: 7902474Publisher: Amsterdam: Elsevier/North-Holland
Institutions: Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
Alkaline-soluble antitumor polysaccharide was prepared from the cell wall of the mushroom Flammulina velutipes. The backbone(s) of the polysaccharide is mainly composed of β-(1→3)-D-linked glucose and its molecular weight was estimated to be about 200 kD. The polysaccharide was found to be non-toxic by brine shrimp assay. When injected into mice intraperitoneally, the polysaccharide triggered proliferation of splenic lymphocytes and also vascular dilation and hemorrhage (VDH) response. The polysaccharide exhibited potent anti-tumor activity against sarcoma SC-180 in vivo but not in vitro.
mushroom, immunostimulation, Flammulina velutipes, anti-tumor polysaccharide
Structure type: homopolymer ; 200000
Location inside paper: SFA1, β-(1→3)-D-glucan
Compound class: EPS, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: TLC, statistical analysis, dialysis, toxicity assays, phenol-sulfuric acid assay, antitumor activity assay, Congo Red assay, aniline blue assay, HCl hydrolysis, lymphocyte proliferation assay
Biological activity: SFA1 polysaccharide exhibited potent anti-tumor activity against sarcoma SC-180 in vivo (with inhibition ratio of 94.1% at 15 mg/kg dose) but not in vitro.
Comments, role: molecular weight of SFA1 fraction was estimated to be greater than or equal to 200 kD
NCBI Taxonomy refs (TaxIDs): 38945Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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