Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: sclerotium
Publication DOI: 10.1016/j.carbpol.2009.05.019Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Zhang L <lnzhang
public.wh.hb.cn>
Institutions: Department of Chemistry, Wuhan University, Wuhan, China, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
(1→3)-β-D-Glucan isolated from Poria cocos was phosphorylated to obtain a series of phosphorylated derivatives. Their structures, weight-average molecular weights (Mw), and chain conformation were studied by 13C NMR, 31P NMR, static laser light scattering and viscometry. The experimental results revealed that the phosphorylated glucan existed as relatively extended flexible chain in 0.15 M NaCl aqueous solution, and exhibited relatively strong inhibition against S-180 tumor cell in vitro and in vivo. In vivo, the fractions with relatively high molecular weight at low dosage exhibited stronger anti-tumor activities. The results revealed that the molecular weights and molecular conformation could influence the anti-tumor activities. The molecular weight ranging from 26000 to 268000 and the extended chain conformation were beneficial to enhance the anti-tumor activity, as a result of the increasing of the interaction between polysaccharide and immune system.
molecular weight, Chain conformation, (1→3)-β-D-glucan, anti-tumor activity, phosphorylated derivative
Structure type: homopolymer
Location inside paper: PCS3-II, (1→3)-β-D-glucan in text, fig. 1b
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, phosphorylation, antitumor activity assay, centrifugation
Comments, role: NMR data obtained for phosphorylated derivatives of PCS3-II was in accordance with results reported in [Williams et al., 1991]
NCBI Taxonomy refs (TaxIDs): 81056Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 293(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.71 73.54 86.89 69.10 77.03 61.57
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.71 | 73.54 | 86.89 | 69.10 | 77.03 | 61.57 |
|
There is only one chemically distinct structure:
Found 
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(later renamed to: Wolfiporia cocos)