Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 28278657Publication DOI: 10.1080/14786419.2017.1285302Journal NLM ID: 101167924Publisher: Milton Park, UK : Taylor & Francis Health Sciences
Correspondence: Assis SA <sandrinhaassis
yahoo.com.br>; Assis SA <sandraassis
uefs.br>
Institutions: Departamento de Saúde, Laboratório de Enzimologia e Tecnologia de Fermentação, Universidade Estadual de Feira de Santana (UEFS), Feira de Santana, Brazil, Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Campus Universitário de Ondina, Salvador, Brasil, Departamento de Química Geral de Inorgânica, Universidade Federal da Bahia - UFBA, Salvador, Brasil, Departamento de Saúde, Laboratório de Bioprospecção Vegetal (LABIV), Universidade Estadual de Feira de Santana (UEFS), Feira de Santana, Brazil, Departamento de Saúde, Universidade Estadual de Feira de Santana (UEFS), Feira de Santana, Brasil
The α-D-glucans are worldwide acknowledged as powerful immune system stimulants found in several sources; however, the fungal-derived sources appear to respond with higher activity. The present study has investigated polysaccharide production in Moniliophthora perniciosa. The dry biomass was subjected to thermal treatment in alkaline solution after fermentation. The biopolymers dissolved in this solution were precipitated after three volumes of absolute ethanol were added to the supernatant. The pure polysaccharide MPS1 was obtained through molecular exclusion chromatography using the Sephacryl S-200 column. The HPLC-RI analysis showed that MPS1 was a glucose homopolysaccharide. Nuclear magnetic resonance (NMR) spectra indicated the α-form as the anomeric carbon configuration in glucose residue. The structure of the polysaccharide was further confirmed as (1→4)-α-D-glucan through the chemical shift of C4. The molecular weight of MPS1 was 31.0 kDa.
polysaccharide, extraction, purification, characterisation, Moniliophthora perniciosa
Structure type: homopolymer ; 31000
Location inside paper: abstract, MPS1
Compound class: glucan
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, acid hydrolysis, FTIR, HPLC, HSQC, HPLC-RI, phenol-sulfuric acid method
Biological activity: MPS1 has shown capacity to make macrophages produce cytokines such as IL-1, IL-2, IL-6 and TNF-α; the linear connections with predominantly (1→4) may show good activity against sarcoma 180 and good immunomodulatory activity
NCBI Taxonomy refs (TaxIDs): 153609Reference(s) to other database(s): GTC:G05740LL
Show glycosyltransferases
NMR conditions: in 86%D2O / 14%DMSO at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 99.9 71.8 73.1 78.6 71.4 60.3
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDGlcp 5.2 3.8 3.65 3.3 3.3 3.71
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDGlcp 99.9/5.2 71.8/3.8 73.1/3.65 78.6/3.3 71.4/3.3 60.3/3.71
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDGlcp | 5.2 | 3.8 | 3.65 | 3.3 | 3.3 | 3.71 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 99.9 | 71.8 | 73.1 | 78.6 | 71.4 | 60.3 |
|
There is only one chemically distinct structure:
Found 
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(previously named: Crinipellis perniciosa CCMB 0257, Marasmius perniciosus CCMB 0257)