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Aspergillus fumigatus

(previously named: Sartorya fumigata, Aspergillus fumigates; later renamed to: Neosartorya fumigata)
(NCBI TaxID 746128, species name lookup)

Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)
Associated disease: infection due to Aspergillus fumigatus [ICD11: XN5Z7

]

NCBI PubMed ID: 29081774
Publication DOI: 10.3389/fimmu.2017.01273
Journal NLM ID: 101560960
Publisher: Lausanne: Frontiers Research Foundation
Correspondence: Paulovičová E <ema.paulovicova

savba.sk>; Nifantiev NE <nen

ioc.ac.ru>
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Department of Immunochemistry of Glycoconjugates, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia, Department of Clinical Immunology and Allergy, Oncology Institute of St. Elisabeth, Bratislava, Slovakia

The study is oriented at the in vitro evaluation of the immunobiological activity and efficacy of synthetically prepared isomeric pentasaccharides representing fragments of Aspergillus fumigatus cell-wall galactomannan and containing β-(1→5)-linked tetragalactofuranoside chain attached to O-6 (GM-1) or O-3 (GM-2) of a spacer-armed mannopyranoside residue. These compounds were studied as biotinylated conjugates which both demonstrated immunomodulatory activities on the RAW 264.7 cell line murine macrophages as in vitro innate immunity cell model. Immunobiological studies revealed time- and concentration-dependent efficient immunomodulation. The proliferation of RAW 264.7 macrophages was induced at higher concentration (100 µg/mL) of studied glycoconjugates and longer exposure (48 h), with more pronounced efficacy for GM-1. The increase of proliferation followed the previous increase of IL-2 production. The cytokine profile of the macrophages treated with the glycoconjugates was predominantly pro-inflammatory Th1 type with significant increase of TNFα, IL-6, and IL-12 release for both glycoconjugates. The RAW 264.7 macrophages production of free radicals was not significantly affected by glycoconjugates stimulation. The phagocytic activity of RAW 264.7 cells was reduced following GM-1 treatment and was significantly increased after 24 h stimulation with GM-2, contrary to 48 h stimulation. Moreover, the synthetically prepared galactomannoside derivatives have been evaluated as efficient serodiagnostic antigens recognized by specific Ig isotypes, and significant presence of specific IgM antibodies in serum of patients suffering from vulvovaginitis was observed.

mannan, Galactomannan, cytokines, Candida, Aspergillus, RAW 264.7

Structure type: structural motif or average structure
Location inside paper: FIGURE 1(GM-native)
Compound class: galactomannan
Contained glycoepitopes: IEDB_130701,IEDB_136095,IEDB_136104,IEDB_137472,IEDB_140116,IEDB_141795,IEDB_141830,IEDB_141834,IEDB_143632,IEDB_144983,IEDB_149137,IEDB_152206,IEDB_164480,IEDB_190606,IEDB_76933,IEDB_885812,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72

Comments, role: hypothetical structure of the galactomannan secreted by A. fumigatus

Related record ID(s): 43025, 43026
NCBI Taxonomy refs (TaxIDs): 746128
Reference(s) to other database(s): GTC:G30542CA
Show glycosyltransferases

There is only one chemically distinct structure:

*O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@H]1[C@@H](O[C@@H]2O[C@@H]([C@@H](CO)O[C@@H]3O[C@@H]([C@@H](CO)O[C@@H]4O[C@@H]([C@@H](CO)O[C@@H]5O[C@@H]([C@H](O)CO)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@H]1[C@@H](O[C@@H]2O[C@@H]([C@@H](CO)O[C@@H]3O[C@@H]([C@@H](CO)O[C@@H]4O[C@@H]([C@@H](CO)O[C@@H]5O[C@@H]([C@H](O)CO)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1*

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at bDGalf: variable unit count (3-4) was assumed to be 3
Warning = sub-repeat ending at bDGalf: variable unit count (3-4) was assumed to be 3