Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperPublication DOI: 10.1016/j.carbpol.2011.06.077Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: jruiz
ira.cinvestav.mx
Institutions: Departamento de Ingeniería Genética, Unidad Irapuato Gto, Centro de Investigación y de Estudios Avanzados del IPN, Mexico, Mexico, Departamento de Biotecnología y Bioquímica, Unidad Irapuato Gto, Centro de Investigación y de Estudios Avanzados del IPN, Mexico, Mexico, Facultad de Ciencias Biológicas,Universidad de Nuevo León, Monterrey, Mexico
Linear (1→3)-β-D-glucans and side-chain-branched β-D-glucans are major constituents of capsular materials, with roles in bacterial aggregation, virulence and carbohydrate storage; whereas branched (1→3),(1→6)-β-D-glucans constitute the most abundant components in the cell walls of fungi. In the present manuscript we describe the chemical characterization of a linear β-D-glucan secreted by mutants of the Basidiomycota biotrophic fungus Ustilago maydis affected in the Pal/Rim pathway involved in pH responses, that in contrast is not made by the wild type strain, at least in measurable amounts. The polysaccharide was obtained from the culture medium of a Δrim13::Cbx mutant of the fungus by ethanol precipitation, and analyzed using FT-IR spectroscopy, NMR spectroscopy in solid and liquid states, HPAEC-PAD chromatography, thin layer chromatography, enzymatic digestion and immunodetection. Our data revealed that, according to its characteristics, the polysaccharide is a (1→3)-β-D-glucan, and that the mutants used represent a relevant resource of this polysaccharide with wide applications.
polysaccharide, (1→3)-β-D-glucan, Ustilago maydis, Pal/Rim pathway
Structure type: homopolymer
Location inside paper: p.1648
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, TLC, acid hydrolysis, Western blotting, FTIR, NMR-1D, enzymatic digestion, HPAEC-PAD, extraction
Enzymes that release or process the structure: (1→3)-β-D-glucanase
Comments, role: 1H NMR in D2O; 13C NMR in the solid state
NCBI Taxonomy refs (TaxIDs): 5270Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.755 74.447 86.170 68.585 74.447 62.333
1H NMR data: present in publication
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.755 | 74.447 | 86.170 | 68.585 | 74.447 | 62.333 |
|
There is only one chemically distinct structure:
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