Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
Publication DOI: 10.1039/c0cy00057dJournal NLM ID: 101636664Publisher: Cambridge: Royal Society of Chemistry
Correspondence: Ferreira TF <tatianafelix
ufrj.br>
Institutions: School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
In this work, a simple method was developed to increase cell wall porosity and to achieve partial hydrolysis of β-glucan. First, the cells were permeabilized using ethanol under agitation. Then the cells were treated with glass beads to increase cell wall porosity and subjected to an enzymatic treatment to degrade glycogen at 65 °C for 30 minutes. Finally, the cells were incubated at 57 °C for 2 hours to partially degrade β-glucan. Whole cells, permeabilized cells and permeabilized cells with partially hydrolyzed β-glucan were evaluated by optical microscopy and transmission electron microscopy. From the images of permeabilized cells with partially hydrolyzed β-glucan it was possible to visualize structures named "ghosts", which are alike along microcapsules containing β-glucan. These structures can be used to aggregate β-glucan in food industrialized products.
hydrolysis, β-glucan
Structure type: homopolymer ; n=1500
Location inside paper: p.1068, first column, third paragraph
Trivial name: β-glucan, 1-3-β-glucan, curdlan
Compound class: O-polysaccharide, cell wall polysaccharide, D-glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: acid hydrolysis, enzymatic digestion, cell growth, TEM, glucose oxidase method
Enzymes that release or process the structure: A7255-amyloglucosidase
Comments, role: β-glucan main chain
Related record ID(s): 43366
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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