Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 22032207Publication DOI: 10.1186/1756-0500-4-454Journal NLM ID: 101462768Publisher: London: Biomed Central, 2008
Correspondence: hubert.becker
polytechnique.edu
Institutions: Laboratoire d’Optique et Biosciences, INSERM, CNRS, Ecole Polytechnique, Palaiseau, France, UPMC Univ Paris 06, CNRS UMR, Laboratoire des Biomolécules, Paris, France, Université Paris-Diderot, Paris, France
Background: To explore chitin synthesis initiation, the effect of addition of exogenous oligosaccharides on in vitro chitin synthesis was studied. Oligosaccharides of various natures and lengths were added to a chitin synthase assay performed on a Saccharomyces cerevisiae membrane fraction. Findings. N-acetylchito-tetra, -penta and -octaoses resulted in 11 to 25% [14C]-GlcNAc incorporation into [14C]-chitin, corresponding to an increase in the initial velocity. The activation appeared specific to N-acetylchitooses as it was not observed with oligosaccharides in other series, such as β-(1,4), β-(1,3) or α-(1,6) glucooligosaccharides. Conclusions: The effect induced by the N-acetylchitooses was a saturable phenomenon and did not interfere with free GlcNAc and trypsin which are two known activators of yeast chitin synthase activity in vitro. The magnitude of the activation was dependent on both oligosaccharide concentration and oligosaccharide size.
Oligosaccharides, glycosyltransferase, chitin synthase, polymerization activation, polysaccharide synthase
Structure type: homopolymer
Trivial name: chitin
Compound class: O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, chitin
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: TLC, radiolabeling, enzymatic digestion, enzymatic assay
Enzymes that release or process the structure: chitinase
Related record ID(s): 41640, 42638, 45260, 45262, 45281, 45287, 45290, 46290, 46291, 46292, 46304, 46323, 46396, 46404
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G97099AY, CCSD:
46067, CBank-STR:5851, GenDB:KF905651
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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