Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wallAssociated disease: sporotrichosis [ICD11:
1F2J 
, ICD11:
XN6GM ];
infection due to Sporothrix schenckii [ICD11:
XN6GM ]
The structure was elucidated in this paperNCBI PubMed ID: 558825Publication DOI: 10.1016/S0008-6215(00)84440-7Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Prairie Regional Laboratory, National Research Council, Saskatoon, SK, Canada, Departamento de Microbiologia Geral, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil
Studies were continued on the 13C and proton nuclear magnetic resonance spectra of a series of mannose-containing polysaccharides formed by various strains of the closely related fungal species, Sporothrix schenckii and Ceratocystis stenoceras, and the criteria for their differentiation redefined. Exocellular filtrates of C. stenoceras 1099.40 contain mainly a galactan, with smaller proportions of a rhamnomannan having single-unit α-L-rhamnopyranosyl side-chains (1), an acidic rhamnomannan (2) containing glucuronic acid, and amylose. The mono-rhamnomannan was characterized by comparison of its 13C spectrum with that of authentic polysaccharide. The acidic rhamnomannan was characterized partly with the aid of 13C n.m.r spectroscopy; in the process, fragments obtained by partial hydrolysis were used to assign signals of C-1 and other O-glycosylated 13C nuclei. Other 13C signals in spectra of polysaccharides from C. stenoceras and S. schenckii were assigned following tracer experiments that used media containing glucoses labeled with deuterium and 13C, and by analogy with 13C signal-displacement effects that are known to occur on O-glycosylation and O-methylation of hydroxyl groups. Evidence was obtained for the presence of structures 3,4, and 5 in exocellular polysaccharide of S. schenckii. These structures give rise to C-1 signals that were assigned by reference to those of (1→4) linked α-D-mannopyranose di- and tri-saccharides.
rhamnomannan, Sporothrix schenckii, Ceratocystis stenoceras
Structure type: structural motif or average structure
Location inside paper: p.26 (3-5)
Compound class: EPS, cell wall polysaccharide, rhamnomannan
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_140116,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: 13C NMR, methylation, acid hydrolysis, GLC, Smith degradation, paper chromatography, ion-exchange chromatography, optical rotation measurement, cell growth, ethanol precipitation
Related record ID(s): 43422, 43423, 43427, 43428, 43489
NCBI Taxonomy refs (TaxIDs): 1397361Reference(s) to other database(s): GTC:G95004KO
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error
(later renamed to: Sporothrix schenckii 1099-18)