Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wallAssociated disease: sporotrichosis [ICD11:
1F2J 
, ICD11:
XN6GM ];
infection due to Sporothrix schenckii [ICD11:
XN6GM ]
NCBI PubMed ID: 1276152Publication DOI: 10.1021/bi00656a028Journal NLM ID: 0370623Publisher: American Chemical Society
Institutions: Prairie Regional Laboratory, National Research Council, Saskatoon, SK, Canada, Departamento de Microbiologia Geral, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil, Department of Biochemistry, School of Medicine, Texas Tech University, Lubbock, USA
The alkali-extractable polysaccharides from different morphological types of two Sporothrix schenckii strains (1099.12 and 1099.18) were investigated. Dissociation of morphological phase transition and temperature effects was possible in a synthetic medium which produced cultures with 100% yeast forms either at 25°C or at 37°C. Only rhamnomannans with single-unit α-L-rhamnopyranosyl side chains were formed by the yeast forms irrespective of the incubation temperature. The higher temperature inhibited formation of 4-O- and 2,4-di-O-substituted α-D-mannopyranose units in the rhamnomannan. An apparently unsporulated mycelium culture of one S. schenckii strain (1099.12) synthesized a galactomannan whose structure was partially determined by methylation analysis and by proton and 13C nuclear magnetic resonance spectroscopy. In another strain (1099.18), a mannan was excreted in the medium of an apparently conidia-less mycelial form at 25°C with short incubation. Its structure was also partially determined. An apparent mixture of this mannan and a rhamnomannan rich in α-L-rhamnopyranosyl-(1 leads to 2)-α-L-rhamnopyranose side chains formed in these cultures on prolonged incubation. The proportion of the excreted rhamnomannan increased as the mycelium sporulated and conidia were more numerous. Mannans or galactomannans may be transient polysaccharides in the young mycelium of S. schenckii. As the culture develops, rhamnomannans are formed in amounts usually masking the presence of other mannose-containing polysaccharides. It is suggested that in S. schenckii different polysaccharides are formed with side chains containing different proportions of rhamnose, mannose, or galactose, as a function of morphological differentiation.
polymorphism, Sporothrix schenckii
Structure type: structural motif or average structure
Location inside paper: p.2424 (I, II)
Compound class: EPS, cell wall polysaccharide, rhamnomannan
Contained glycoepitopes: IEDB_130701,IEDB_133754,IEDB_136105,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_225177,IEDB_76933,IEDB_885823,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, methylation, acid hydrolysis, GLC, Smith degradation, cell growth, precipitation, phenol-sulfuric acid assay
Related record ID(s): 43426, 43427
NCBI Taxonomy refs (TaxIDs): 29908Reference(s) to other database(s): GTC:G13333EQ
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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