Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wallAssociated disease: sporotrichosis [ICD11:
1F2J 
, ICD11:
XN6GM ];
infection due to Sporothrix schenckii [ICD11:
XN6GM ]
NCBI PubMed ID: 804993Publication DOI: 10.1016/S0008-6215(00)82671-3Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Departments of Dermatology and Biochemistry, College of Physicians and Surgeons, Columbia University, New York, USA, Department of Biochemistry, Texas Tech University School of Medicine, Lubbock, Texas, USA, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil
Antisera were prepared in rabbits against the human pathogenic yeast Sporothrix schenckii (strain 1099.12) grown at two different temperatures (25°C and 37°C). Precipitation and inhibition data showed that the former serum had a specificity directed against α-L-Rhap-(1→2)-α-L-Rhap-(1→3)-D-Man-(1→ determinants, whereas the latter had a broad specificity in which α-L-rhamnosyl or α-L-Rhap-(1→3)-D-Man- was the immunodominant structure. These results are consistent with data on the structures of the L-rhamno-D-mannans isolated from the organism grown at the two different temperatures. Human sera from patients with sporotrichosis were shown to have different specificities resembling the specificities developed in the rabbits. The rabbit antisera were also used to examine the cross-reactivity with L-rhamno-D-mannans from species of the genus Ceratocystis, which is reputed to include the ascigerous (perfect) state of S. schenckii. Polysaccharides from four species of Ceratocystis grown at 25°C reacted with the antisera in a manner resembling that of the L-rhamno-D-mannan from S. schenckii grown at 37°C. This is in accord with earlier data that showed that only S. schenckii, of the species studied, produces a polysaccharide with large amounts of α-L-Rhap-(1→2)-α-L-Rhap-(1→ side-chains when grown at 25°C.
immunochemical studies
Structure type: polymer biological repeating unit
Location inside paper: scheme 1, SS12-25
Compound class: EPS, cell wall polysaccharide, rhamnomannan
Contained glycoepitopes: IEDB_130701,IEDB_133754,IEDB_136105,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_225177,IEDB_76933,IEDB_885823,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Methods: 13C NMR, GLC, biological assays, paper chromatography, immunochemical methods, extraction, optical rotation measurement, methylation analysis, partial hydrolysis
Biological activity: rabbit serum 227, human serum Lop, human serum Guil, and 228 precipitate very strongly with this polysaccharide
Comments, role: dirhamnosil side chain is typical for culture growing at 25C
Related record ID(s): 43432, 43433
NCBI Taxonomy refs (TaxIDs): 29908Reference(s) to other database(s): GTC:G13333EQ
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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