Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wallAssociated disease: sporotrichosis [ICD11:
1F2J 
, ICD11:
XN6GM ];
infection due to Sporothrix schenckii [ICD11:
XN6GM ]
The structure was elucidated in this paperNCBI PubMed ID: 4748942Journal NLM ID: 0246127Publisher: American Society for Microbiology
Institutions: Departments of Dermatology and Biochemistry, College of Physicians and Surgeons, Columbia University, New York, USA, Prairie Regional Laboratory, National Research Council, Saskatoon, Saskatchewan, Canada
Rhamnomannans from several isolates of Sporothrix schenckii and Ceratocystis species were compared. No major differences emerged in the analysis of the carbohydrate composition of polysaccharides from either species. Methylation analysis for the identification of the glycosidic linkages present in the polysaccharides showed that they all were very similar with minor differences being observed among strains and between polysaccharides obtained at 25 and 37 C. Partially methylated derivatives were identified by gas-liquid chromatography and mass spectrometry. Their proportions suggest that polysaccharides from S. schenckii and C. stenoceras have a general structure similar to that of C. ulmi: a (1→6)-linked α-D-mannopyranosyl main chain substituted in the 3-positions by α-L-rhamnopyranosyl and in many cases by α-L-rhamnopyranosyl (1→2)-L-rhamnopyranosyl side chains. Differences among polysaccharides resided in the proportions of the dirhamnosyl side chains and of 4-O-substituted and 2, 4-di-O-substituted mannose units. Increased proportions of dirhamnosyl side chains were observed in the polysaccharides from S. schenckii strains and from a pathogenic variant of C. stenoceras grown at 25 C. Proton magnetic resonance spectra (H-1 region) of the rhamnomannans showed that S. schenckii strains could be placed in two different groups similar but not identical to any Ceratocystis species. These spectra were very close to those of the polysaccharides from the C. clavata and C. ambrosia groups of Ceratocystis species.
rhamnomannan, sporotrichosis
Structure type: structural motif or average structure
Location inside paper: p.691, fig.2
Compound class: EPS, cell wall polysaccharide, rhamnomannan
Contained glycoepitopes: IEDB_130701,IEDB_133754,IEDB_136105,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_225177,IEDB_76933,IEDB_885823,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Methods: 1H NMR, GC-MS, acid hydrolysis, GLC, optical rotation measurement, methylation analysis, cell growth, precipitation, phenol-sulfuric acid assay
Related record ID(s): 43427
NCBI Taxonomy refs (TaxIDs): 29908,
1397361,
5173,
5174Reference(s) to other database(s): GTC:G13333EQ
Show glycosyltransferases
NMR conditions: in D2O at 343(H) K
[as TSV]
1H NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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