Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 21924407Publication DOI: 10.1016/j.carres.2011.08.019Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Islam SS <sirajul_1999
yahoo.com>
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Plant Molecular and Cellular Genetics Section, Bose Institute, Kolkata, India
Three polysaccharide fractions (PS-I, PS-II, and PS-III) were isolated from the aqueous extract of a hybrid mushroom obtained through backcross mating of a somatic hybrid mushroom PfloVv12 (Sterile line) with Volvariella volvacea. PfloVv12 was obtained through protoplast fusion of Pleurotus florida and V. volvacea. PS-I was identified as 1,6-β glucan. PS-II and PS-III were identified as mannoglucogalactan but differing in molecular weights only. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, and NMR experiment ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structures of these polysaccharides were established as: PS-I is -6)bDGlcp(1-, PS-II and PS-III is -6)[bDManp(1-2)]aDGlcp(1-6)aDGalp(1-.
structure, polysaccharide, NMR spectroscopy, Hybrid mushroom
Structure type: homopolymer
Location inside paper: p. 2452, Table I, PS-I
Trivial name: pustulan, β-1,6-glucan, β-1,6-D-glucan, β(1-6)-D-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-(1,6)-glucan, lasiodiplodan, β-(1,6)-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-1,6-glucan, β-(1,6)-glucan, pustulan, β-(1→6)-glucan PCPS, water-soluble glucan (PS-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glycoprotein, glucan, polysaccharide, cell wall glucoprotein
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, periodate oxidation, GLC-MS, NMR-2D, acid hydrolysis, GLC, extraction, optical rotation measurement, methylation analysis, HMBC, DEPT, NOESY
Comments, role: Current organism is a hybrid of PfloVv12 and Volvariella volvacea. PfloVv12 is a hybrid of Pleurotus florida and Volvariella volvacea. 1H NMR is referenced to HDO at 4.74, 13C NMR is referenced to acetone at 31.05.
Related record ID(s): 41577, 43611
NCBI Taxonomy refs (TaxIDs): 188765,
36659Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863, CCSD:
50854, CBank-STR:4234
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.2 73.5 76.0 70.0 75.3 67.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.50 3.31 3.48 3.41 3.62 3.85-4.19
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 103.2/4.50 73.5/3.31 76.0/3.48 70.0/3.41 75.3/3.62 67.2/3.85-4.19
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.50 | 3.31 | 3.48 | 3.41 | 3.62 | 3.85
4.19 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.2 | 73.5 | 76.0 | 70.0 | 75.3 | 67.2 |
|
There is only one chemically distinct structure:
Found 
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(previously named: Pleurotus sp. Florida x Volvariella volvacea)