Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: mycelium
The structure was elucidated in this paperPublication DOI: 10.1016/j.carbpol.2011.03.043Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Wu JY <bcjywu
polyu.edu.hk>
Institutions: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China, Department of Applied Biology and Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hong Kong, China, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China
Two novel polysaccharides, WIPS and AIPS, were isolated and fractionated respectively from hot water and alkaline extracts of the mycelial biomass of a medicinal fungus Cordyceps sinensis (strain Cs-HK1). Through analytical and degradation experiments, WIPS and AIPS were characterized as α-d-glucans with a backbone of (1→4)-linked α-d-Glcp (>60%) and very similar molecular weights (Mw: WIPS 1180 kDa; AIPS 1150 kDa). WIPS had a short branch of (1→6)-linked α-d-Glcp (∼14%), but AIPS was a linear glucan, distinctive from the branched structures of most glucans from medicinal fungi. In aqueous alkaline solutions, both AIPS and WIPS exhibited a random coil structure with the similar conformational parameters but significantly different polydispersity indexes, 0.19 versus 0.37. AIPS exhibited much more significant antitumor and immuno-stimulatory effects than WIPS in animal tests on melanoma tumor-bearing mice.
conformation, structure, polysaccharides, antitumor activity, medicinal fungus
Structure type: homopolymer ; 1150000
Location inside paper: AIPS, p.756, table 2
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, GC, biological assays, FTIR, GPC, ion-exchange chromatography, extraction, optical rotation measurement, CC, dynamic light scattering
Biological activity: exhibited moderate antitumor activity with about 28% inhibition of the tumor growth; caused a slight inhibition of the animal growth; showed an enhancement effect on the T-cell proliferation or viability at high significance levels
Comments, role: alkaline extract; average particle size in aqueous solution/dispersion were distributed in a relatively narrow range around 37.9 nm
Related record ID(s): 43671, 43691, 43695, 44831
NCBI Taxonomy refs (TaxIDs): 72228Reference(s) to other database(s): GTC:G05740LL
Show glycosyltransferases
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 99.28 71.02 72.76 76.22 70.62 59.94
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 99.28 | 71.02 | 72.76 | 76.22 | 70.62 | 59.94 |
|
There is only one chemically distinct structure:
Found 
report error
(later renamed to: Ophiocordyceps sinensis Cs-HK1)