Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperPublication DOI: 10.1016/j.carbpol.2011.05.031Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: lnzhang
public.wh.hb.cn, linazhangwhu
gmail.com
Institutions: Department of Chemistry, Wuhan University, Wuhan, China, Glyn O. Philips Hydrocolloid Research Centre, School of Bioengineering, Hubei University, Hubei, China, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
Five water-soluble heteropolysaccharides designated GL-I to GL-V, were isolated from the cultured fruit body of Ganoderma lucidum. Using a range of techniques the samples are shown to be heteropolysaccharides, mainly composed of glucose, galactose, mannose, arabinose with the relatively low weight-average molecular weights of 61000 to 184000. The amount of uronic acid present is low. As successive isolations proceed to give GL-I to GL-V, the content of glucan increased, the content of galactan and mannan decreased, and the branching decreased from high to near linear. GL-I is most branched of the heteropolysaccharides with a 27.0% degree of branching, whereas GL-V is mainly a linear glucan. Due to their branched chains samples GL-I to GL-III are in a compact coil conformation in 0.9% NaCl at 25 °C, whereas GL-V has a slightly flexible chain. Atomic force microscopy images confirmed the spherical shape of samples GL-I to GL-III in water.
structure, polysaccharide, Ganoderma lucidum, anti-tumor activities, degree of branching, water-soluble
Structure type: homopolymer ; 78000-103000
Location inside paper: GL-V, p.847, fig.3, fig. 4
Trivial name: 1,3-β-glucan
Compound class: glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, GC-MS, GC, FTIR, NMR-1D, light scattering, viscosity measurement, extraction, acetylation, atomic force microscopy, SEC, CC
Comments, role: backbone chain; very little branch chains; water soluble
Related record ID(s): 41810, 42046, 42047, 42048, 42049
NCBI Taxonomy refs (TaxIDs): 5315Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
NMR conditions: in D2O
1H NMR data: present in publication
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13C NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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