Sparassis crispa and Phellinus linteus are edible/medicinal mushrooms that have remarkably high contents of β-(1→3)-D-glucan, which acts as a biological response modifier, but difficulty in cultivating the fruiting bodies and extraction of β-D-glucan have restricted detailed studies. Therefore, a novel process for nanoparticle extraction of Sparan, the β-D-glucan from Sparassis crispa, and Phellin, the β-D-glucan from Phellinus linteus, has been investigated using insoluble tungsten carbide as a model for nanoknife technology. This is the first report showing that the nanoknife method results in high yields of Sparan (70.2%) and Phellin (65.2%) with an average particle size of 150 and 390 nm, respectively. The extracted Sparan with β-(1→3) linkages showed a remarkably high water solubility of 90% even after 10 min of incubation at room temperature. Therefore, it is likely that this nanoknife method could be used to produce β-D-glucan for food, cosmetic, and pharmaceutical industries.

extraction, Sparassis crispa, nanometer-sized β-glucan, nanoknife, Phellinus linteus, Sparan, Phellin

13C NMR data:
missing...

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

*O[C@@H]1[C@@H](O)[C@H](*)O[C@H](CO)[C@H]1O

162.141 g/mol (C₆H₁₀R₂O₅, R = next and previous repeats, not counted in mol weight)