Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 22652093Publication DOI: 10.1016/j.carres.2012.04.007Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Islam SS <sirajul_1999
yahoo.com>
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India, Department of Chemistry, PG Centre, Panskura Banamali College, Midnapore, India
Two immunostimulating β-glucans, PS-I (water soluble) and PS-II (water insoluble) isolated from hot water extract of the fruiting bodies of an edible mushroom Termitomyces robustus var. showed significant macrophage, splenocyte, and thymocyte activation. On the basis of total hydrolysis, methylation analysis, periodate oxidation, and NMR experiments (1H, 13C, DQF-COSY, TOCSY, DEPT-135, HSQC, and HMBC), the structure of the repeating unit of the polysaccharides is established as: PS-I: Structure 1; PS-II: Structure 2.
NMR spectroscopy, immunostimulation, mushroom polysaccharide, Termitomyces robustus var., b-glucan
Structure type: homopolymer ; 182000
Location inside paper: Table 1, PS-I
Trivial name: 1-6-β-glucan
Compound class: O-polysaccharide, D-glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, acid hydrolysis, GLC, paper chromatography, FTIR, gel chromatography, optical rotation measurement, cytokine production, HMBC, MTT, HSQC, splenocyte proliferation assay, thymocyte proliferation assay
Biological activity: stimulation of mice splenocytes, thymocytes and peritoneal macrophages proliferation
Comments, role: water soluble glucan
Related record ID(s): 43786
NCBI Taxonomy refs (TaxIDs): 201773Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.4 73.4 76.0 69.9 75.3 69.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.50 3.31 3.48 3.46 3.61 3.83-4.20
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 103.4/4.50 73.4/3.31 76.0/3.48 69.9/3.46 75.3/3.61 69.2/3.83-4.20
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.50 | 3.31 | 3.48 | 3.46 | 3.61 | 3.83
4.20 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.4 | 73.4 | 76.0 | 69.9 | 75.3 | 69.2 |
|
There is only one chemically distinct structure:
Found 
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