Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 22322111Publication DOI: 10.1016/j.ijbiomac.2012.01.031Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Islam SS <sirajul_1999
yahoo.com>
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Division of Plant Biology, Bose Institute, Kolkata, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India
Two different glucans (PS-I and PS-II) were isolated from the alkaline extract of the fruiting bodies of an edible mushroom, Pleurotus florida blue variant and the PS-I showed macrophage, splenocyte and thymocyte activations. On the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies (1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC), the structure of the repeating unit of these polysaccharides were established.
structure, NMR spectroscopy, mushroom polysaccharide, Immunoactivation, Pleurotus florida blue variant
Structure type: homopolymer ; 202000
Location inside paper: Table 1, Table 2, p.596, PS-I
Trivial name: 1-6-β-glucan
Compound class: O-polysaccharide, cell wall polysaccharide, D-glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, GLC-MS, NMR-2D, GLC, paper chromatography, extraction, gel chromatography, optical rotation measurement, generation of reactive oxygen species (ROS), cytokine production, DEPT, MTT, splenocyte proliferation assay, thymocyte proliferation assay
Biological activity: stimulation of mouse splenocytes, thymocytes and peritoneal macrophages proliferation
Comments, role: water soluble glucan
Related record ID(s): 43795
NCBI Taxonomy refs (TaxIDs): 188765Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.4 73.4 75.9 69.9 75.3 69.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.51 3.31 3.48 3.46 3.61 3.84-4.19
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 103.4/4.51 73.4/3.31 75.9/3.48 69.9/3.46 75.3/3.61 69.2/3.84-4.19
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.51 | 3.31 | 3.48 | 3.46 | 3.61 | 3.84
4.19 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.4 | 73.4 | 75.9 | 69.9 | 75.3 | 69.2 |
|
There is only one chemically distinct structure:
Found 
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(previously named: Pleurotus ostreatus var. florida; later renamed to: Pleurotus sp. Florida; Pleurotus sp. Florida blue variant)