Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: (Salmonidae)
Associated disease: vibriosis [ICD11:
XN8RL 
]
The structure was elucidated in this paperNCBI PubMed ID: 9578491Publication DOI: 10.1046/j.1432-1327.1998.2530319.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: eleonora.altman
nrc.ca
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada, Department of Microbiology, College of Biological Sciences, University of Guelph, Canada
Structures of the capsular and O-chain polysaccharides of Vibrio ordalii serotype O:2, the causative agent of vibriosis in salmonid fish, were determined by high-field NMR techniques, mass spectrometric methods and partial hydrolysis. Both polymers were shown to be composed of linear tetrasaccharide repeating units, having the structure: carbohydrate sequence [see text]
Lipopolysaccharide, NMR, capsular polysaccharide, Vibrio ordalii
Structure type: polymer chemical repeating unit
Location inside paper: p.319, abstract, p.323, fig.6, p. 325, fig.8b
Compound class: CPS, O-polysaccharide, O-antigen
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, composition analysis
Related record ID(s): 4463, 4485
NCBI Taxonomy refs (TaxIDs): 28174
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,4,2 Ac
3,4,4,3,2 Fo 164.3
3,4,4,3 xLAla? ? 49.5 18.9
3,4,4,6 NH2
3,4,4 bDGlcpN3NA 102.4 53.9 53.2 75.9 75.9 174.1
3,4,2 Ac
3,4,3 Am 167.3 19.6
3,4 bDGlcpN3NA 102.8 53.5 57.0 79.0 78.3 174.6
3,2 Ac
3,3 Ac
3 aLGalpN3NA 98.1 44.6 50.4 78.3 68.1 175.2
2 Ac
bD6dxylHexpN-4-ulo 101.6 55.2 78.3 94.0 73.8 12.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,4,2 Ac - 1.98
3,4,4,3,2 Fo 8.07
3,4,4,3 xLAla? - 4.40 1.30
3,4,4,6 NH2
3,4,4 bDGlcpN3NA 4.60 3.70 4.03 3.87 3.95 -
3,4,2 Ac - 1.98
3,4,3 Am ? 2.12
3,4 bDGlcpN3NA 4.73 3.84 3.93 3.85 3.79 -
3,2 Ac - 1.98
3,3 Ac - 2.05
3 aLGalpN3NA 5.08 4.40 4.40 4.12 4.82 -
2 Ac - 1.95
bD6dxylHexpN-4-ulo 4.35 3.83 3.72 - 3.50 1.22
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,4,2 Ac NMR TSV error 2: unequal length of 13C and 1H datasets
3,4,4,3,2 Fo 164.3/8.07
3,4,4,3 xLAla? 49.5/4.40 18.9/1.30
3,4,4,6 NH2
3,4,4 bDGlcpN3NA 102.4/4.60 53.9/3.70 53.2/4.03 75.9/3.87 75.9/3.95
3,4,2 Ac NMR TSV error 2: unequal length of 13C and 1H datasets
3,4,3 Am 167.3/? 19.6/2.12
3,4 bDGlcpN3NA 102.8/4.73 53.5/3.84 57.0/3.93 79.0/3.85 78.3/3.79
3,2 Ac NMR TSV error 2: unequal length of 13C and 1H datasets
3,3 Ac NMR TSV error 2: unequal length of 13C and 1H datasets
3 aLGalpN3NA 98.1/5.08 44.6/4.40 50.4/4.40 78.3/4.12 68.1/4.82
2 Ac NMR TSV error 2: unequal length of 13C and 1H datasets
bD6dxylHexpN-4-ulo 101.6/4.35 55.2/3.83 78.3/3.72 73.8/3.50 12.2/1.22
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,4,2 | Ac |
| 1.98 | |
| 3,4,4,3,2 | Fo | 8.07 | |
| 3,4,4,3 | xLAla? |
| 4.40 | 1.30 | |
| 3,4,4,6 | NH2 | |
| 3,4,4 | bDGlcpN3NA | 4.60 | 3.70 | 4.03 | 3.87 | 3.95 |
|
| 3,4,2 | Ac |
| 1.98 | |
| 3,4,3 | Am | ? | 2.12 | |
| 3,4 | bDGlcpN3NA | 4.73 | 3.84 | 3.93 | 3.85 | 3.79 |
|
| 3,2 | Ac |
| 1.98 | |
| 3,3 | Ac |
| 2.05 | |
| 3 | aLGalpN3NA | 5.08 | 4.40 | 4.40 | 4.12 | 4.82 |
|
| 2 | Ac |
| 1.95 | |
| | bD6dxylHexpN-4-ulo | 4.35 | 3.83 | 3.72 |
| 3.50 | 1.22 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,4,2 | Ac | |
| 3,4,4,3,2 | Fo | 164.3 | |
| 3,4,4,3 | xLAla? | ? | 49.5 | 18.9 | |
| 3,4,4,6 | NH2 | |
| 3,4,4 | bDGlcpN3NA | 102.4 | 53.9 | 53.2 | 75.9 | 75.9 | 174.1 |
| 3,4,2 | Ac | |
| 3,4,3 | Am | 167.3 | 19.6 | |
| 3,4 | bDGlcpN3NA | 102.8 | 53.5 | 57.0 | 79.0 | 78.3 | 174.6 |
| 3,2 | Ac | |
| 3,3 | Ac | |
| 3 | aLGalpN3NA | 98.1 | 44.6 | 50.4 | 78.3 | 68.1 | 175.2 |
| 2 | Ac | |
| | bD6dxylHexpN-4-ulo | 101.6 | 55.2 | 78.3 | 94.0 | 73.8 | 12.2 |
|
![](nmr_drawer.py?width=750&height=130&nucleus=C&rotate_labels=false&spectrum=[{"s":164.3,"l":"1","c":[0,0,225]},{"s":49.5,"l":"2","c":[230,0,30]},{"s":18.9,"l":"3","c":[230,0,30]},{"s":102.4,"l":"1","c":[0,165,0]},{"s":53.9,"l":"2","c":[0,165,0]},{"s":53.2,"l":"3","c":[0,165,0]},{"s":75.9,"l":"4","c":[0,165,0]},{"s":75.9,"l":"5","c":[0,165,0]},{"s":174.1,"l":"6","c":[0,165,0]},{"s":167.3,"l":"1","c":[120,0,225]},{"s":19.6,"l":"2","c":[120,0,225]},{"s":102.8,"l":"1","c":[0,192,255]},{"s":53.5,"l":"2","c":[0,192,255]},{"s":57,"l":"3","c":[0,192,255]},{"s":79,"l":"4","c":[0,192,255]},{"s":78.3,"l":"5","c":[0,192,255]},{"s":174.6,"l":"6","c":[0,192,255]},{"s":98.1,"l":"1","c":[220,0,180]},{"s":44.6,"l":"2","c":[220,0,180]},{"s":50.4,"l":"3","c":[220,0,180]},{"s":78.3,"l":"4","c":[220,0,180]},{"s":68.1,"l":"5","c":[220,0,180]},{"s":175.2,"l":"6","c":[220,0,180]},{"s":101.6,"l":"1","c":[100,160,0]},{"s":55.2,"l":"2","c":[100,160,0]},{"s":78.3,"l":"3","c":[100,160,0]},{"s":94,"l":"4","c":[100,160,0]},{"s":73.8,"l":"5","c":[100,160,0]},{"s":12.2,"l":"6","c":[100,160,0]}])
The spectrum also has 1 signal at unknown position (not plotted). |
There is only one chemically distinct structure:
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Found 1 record.
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