Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 9756928Publication DOI: 10.1074/jbc.273.41.26836Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: Munro S <sean
mrc-lmb.cam.ac.uk>
Institutions: Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
The mannan structure found on the N-linked glycans of the yeast Saccharomyces cerevisiae is composed of a long backbone of α-1,6-linked mannose to which are attached branches consisting of two α-1,2-linked mannoses followed by an α-1,3-linked mannose. In the mutants mnn2 and mnn5, the addition of the first and second of these two mannoses, respectively, is defective. In this paper, we report the identification of the genes corresponding to these mutations. The two genes encode closely related proteins with distant homology to the known Mnn1p α-1,3-mannosyltransferase. We show that these proteins are localized in an early compartment of the yeast Golgi and that they are not physically associated with each other or with the two protein complexes known to be involved in synthesizing the α- 1,6-linked backbone. The identification of Mnn2p and Mnn5p allows us to assign Golgi proteins to all of the catalytic steps in S. cerevisiae mannan synthesis.
glycosylation, mannan, Candida albicans, mannosyltransferases, nucleotide sugar
Structure type: homopolymer
Location inside paper: Fig.1, A, mnn2
Trivial name: LAM, mannan, galactomannan, cell wall heteromannan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, N-glycan, cell surface polysaccharide, mannan, D-mannan, galactomannan
Contained glycoepitopes: IEDB_130701,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_76933,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Methods: immunoblotting, gel immunoprecipitation, cell growth, immunofluorescence microscopy, enzymatic assay
Enzymes that release or process the structure: Mnn9p, Van1p
Related record ID(s): 44104, 44105, 44106
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G55317BB, GlycomeDB:
6776, CCSD:
46066, CBank-STR:6345
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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