Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 22942695Publication DOI: 10.3390/ijms13078142Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: Medeiros SD <sarah_farma
hotmail.com>; Cordeiro SL <sara-cordeiro
hotmail.com>; Cavalcanti JE <jessy_ecc
hotmail.com>; Melchuna KM <karinamelchuna
yahoo.com.br>; Lima AM <aleida_maria85
yahoo.com.br>; Filho IA <irami.filho
uol.com.br>; Medeiros AC <aldo
ufrnet.br>; Rocha KB <keyla.rocha
uol.com.br>; Oliveira EM <babethmaia
hotmail.com>; Faria ED <eduardofaria
ufrnet.br>; Sassaki GL <sassaki
ufpr.br>; Rocha HA <hugo
cb.ufrn.br>; Sales VS <vsfsales
hotmail.com>
Institutions: Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil, Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil, University Hospital Onofre Lopes, Department of Surgery, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil, Laboratory of Pathology, Department of Pathology, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil, University Hospital Onofre Lopes, Department of Integrated Medicine, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil, Department of Biochemistry and Molecular Biology, Federal University of Paraná (UFPR), Cento Politécnico S/N, Curitiba, Brazil
Water-insoluble glucan was isolated from the baker's yeast Saccharomyces cerevisiae. The yeast cells were treated with alkali and the residue then with acid. Chemical and NMR (1D and 2D) analyses showed that a linear (1→3)-β-glucan was purified that was not contaminated with other carbohydrates, proteins or phenolic compounds. The effects of the glucan on wound healing were assessed in human venous ulcers by histopathological analysis after 30 days of topical treatment. (1→3)-β-glucan enhanced ulcer healing and increased epithelial hyperplasia, as well as increased inflammatory cells, angiogenesis and fibroblast proliferation. In one patient who had an ulcer that would not heal for over 15 years, glucan treatment caused a 67.8% decrease in the area of the ulcer. This is the first study to investigate the effects of (1→3)-β-glucan on venous ulcer healing in humans; our findings suggest that this glucan is a potential natural biological response modifier in wound healing.
polysaccharide, immunomodulator, yeast, tissue repair, water-insoluble glucan
Structure type: homopolymer
Location inside paper: Fig.1, p.8146, table 1
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, NMR-2D, acid hydrolysis, biological assays, HPLC, extraction, phenol-sulfuric acid assay, DEPT, HSQC
Biological activity: (1→3)-β-glucan enhanced venous ulcer healing and increased epithelial hyperplasia, as well as increased the number of plasmocytes and fibroblast proliferation
Comments, role: details of the 13C NMR referencing are unclear from the paper; although not written, it seems that chemical shifts from acetone (at 32.77 rel. to DSS) were corrected to accord to TMS reference.
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 / DSS at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 102.85 72.94 85.95 68.41 76.26 60.91
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.53 3.32 3.50 3.27 3.27 3.49-3.71
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 102.85/4.53 72.94/3.32 85.95/3.50 68.41/3.27 76.26/3.27 60.91/3.49-3.71
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.53 | 3.32 | 3.50 | 3.27 | 3.27 | 3.49
3.71 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 102.85 | 72.94 | 85.95 | 68.41 | 76.26 | 60.91 |
|
There is only one chemically distinct structure:
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