Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
Publication DOI: 10.1016/S0008-6215(00)81505-0Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Biochemistry, University of Minnesota, St. Paul, MN, USA
The cell-wall D-glucan (αD25=-10° in methyl sulfoxide) of bakers' yeast has been shown by methylation and periodate oxidation studies to be a β-(1→3)-D-glucan having branches at C-6. Hyrdrolysis of the methylated D-glucan (1 mole) yielded 2,3,4,6-tetra- (1 mole), 1,2,4,6-tri- (7 moles), and 2,4-di-O-methyl-D-glucose (1 mole), and trace proportions of 2,3,4-tri-O-methyl-D-glucose, which indicated the presence of a small proportion of (1→6)-linked residues in the molecule. Periodate oxidation of the D-glucan (DP 410), followed by reduction and mild hydrolysis with acid, eliminated one D-glucose residue out of every 9, and yielded the corresponding, degraded D-glucan (DP 150), glycerol, and a small proportion of a 1-O-β-D-glucosylglycerol, which would be derived from the sequence of the D-glucose residues in the glucan molecule represented by the system →6)-Gp-(1→3)-Gp-(1→6)-Gp-(1→. Methylation of the degraded glucan gave material which, on hydrolysis, gave, per mole,2,3,4,6-tetra-(1 mole),2,4,6-tri-(6 moles),and 2,4-di-O-methyl-D-glucose(1 mole). The (1→3)-β-D-glucanase from Rhizopus arrhizus acted on the D-glucan and on the degraded D-glucan to give the same products, viz., D-glucose, laminaribiose, gentiobiose, and higher oligosaccharides. The structure of the cell-wall D-glucan is discussed on the basis of these findings.
cell wall, glucan, Saccharomyces cerevisiae, bakers' yeast, Rhizopus arrhizus
Structure type: fragment of a bigger structure
Location inside paper: p.154, structure 2
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: methylation, periodate oxidation, acid hydrolysis, paper chromatography, enzymatic digestion, extraction, optical rotation measurement, paper electrophoresis, reduction, precipitation, phenol-sulfuric acid assay, evaporation
Related record ID(s): 44294, 44296, 44297, 44298
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G63821UG
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error