Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 11054112Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: sgronow
fz-borstel.de
Institutions: Division of Medical and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany
Heptosyltransferase II, encoded by the waaF gene of Escherichia coli, is a glycosyltransferase involved in the synthesis of the inner core region of lipopolysaccharide. The gene was subcloned from plasmid pWSB33 [Brabetz, W., Müller-Loennies, S., Holst, O. & Brade, H. (1997) Eur. J. Biochem. 247, 716-724] into a shuttle vector for the expression in the gram-positive host Corynebacterium glutamicum. The in vitro activity of the enzyme was investigated in comparison to that of heptosyltransferase I (WaaC) using as a source for the sugar nucleotide donor, ADP-LglyceroDmanno-heptose, a low molecular mass filtrate from a ∆waaCF E. coli strain. Synthetic lipid A analogues varying in the acylation or phosphorylation pattern or both were tested as acceptors for the subsequent transfer of 3-deoxy-Dmanno-oct-2-ulosonic acid (Kdo) and heptose by successive action of Kdo transferase (WaaA), heptosyltransferase I (WaaC) and heptosyltransferase II (WaaF). The reaction products were characterized after separation by TLC and blotting with monoclonal antibodies specific for the acceptor, the intermediates and the final products.
Lipopolysaccharide, biosynthesis, core, functional, characterization, Escherichia, Escherichia coli, monoclonal antibodies, heptosyltransferase, in vitro
Structure type: oligomer
Location inside paper: p.6603
Aglycon: lipid A
Trivial name: inner core region, outer core region
Compound class: core oligosaccharide, LPS
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_140088,IEDB_2189047
Biological activity: serological study using monoclonal antibodies
Enzymes that release or process the structure: heptosyltransferase I and II
Biosynthesis and genetic data: biosynthesis data
Synthetic data: enzymatic
Related record ID(s): 6305, 6430, 24942
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G18751QK, GlycomeDB:
26995
Show glycosyltransferases
There is only one chemically distinct structure:
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