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Aspergillus fumigatus CBS 144-89

(previously named: Sartorya fumigata CBS 144-89, Aspergillus fumigates CBS 144-89, Neosartorya fumigata CBS 144-89)
(Ancestor NCBI TaxID 746128, species name lookup)

Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)
Associated disease: infection due to Aspergillus fumigatus [ICD11: XN5Z7

]

The structure was elucidated in this paper
NCBI PubMed ID: 16002656
Publication DOI: 10.1128/EC.4.7.1308-1316.2005
Journal NLM ID: 101130731
Publisher: American Society for Microbiology
Correspondence: jplatge

pasteur.fr
Institutions: Unité des Aspergillus, Institut Pasteur, Paris, France, Bio-Rad, Clinical Microbiology Division, Steenvoorde, France

Galactofuranose-containing molecules have been repeatedly shown to be important antigens among human fungal pathogens, including Aspergillus fumigatus. Immunogenic galactofuran determinants have been poorly characterized chemically, however. We reported here the characterization of two glycoproteins of A. fumigatus with an N-glycan containing galactofuranose. These proteins are a phospholipase C and a phytase. Chemical characterization of the N-glycan indicates that it is a mixture of Hex(5-13)HexNAc(2) oligosaccharides, the major molecular species corresponding to Hex(6-8)HexNAc(2). The N-glycan contained one galactofuranose unit that was in a terminal nonreducing position attached to the 2 position of Man. This single terminal nonreducing galactofuranose is essential for the immunoreactivity of the N-glycans assessed either with a monoclonal antibody that recognizes a tetra-β-1,5-galactofuran chain of galactomannan or with Aspergillus-infected patient sera.

Structure type: structural motif or average structure ; 1257 (Hex5HexNAc2), 1419 (Hex6HexNAc2), 1581 (Hex7HexNAc2), 1743 (Hex8HexNAc2), 1905 (Hex9HexNAc2), 2067 (Hex10HexNAc2), 2229 (Hex11HexNAc2)
Location inside paper: Fig. 1,2,6
Compound class: N-glycan, galactomannan
Contained glycoepitopes: IEDB_123886,IEDB_130701,IEDB_135813,IEDB_136095,IEDB_136104,IEDB_137340,IEDB_137472,IEDB_137485,IEDB_140116,IEDB_141793,IEDB_141795,IEDB_141807,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_141832,IEDB_141833,IEDB_141834,IEDB_143632,IEDB_144983,IEDB_147453,IEDB_149137,IEDB_149176,IEDB_151079,IEDB_151531,IEDB_152206,IEDB_153212,IEDB_153220,IEDB_164174,IEDB_164480,IEDB_187201,IEDB_190606,IEDB_423157,IEDB_429156,IEDB_548907,IEDB_76933,IEDB_857734,IEDB_885812,IEDB_983930,SB_136,SB_191,SB_196,SB_197,SB_198,SB_33,SB_44,SB_53,SB_67,SB_72,SB_73,SB_74,SB_77,SB_85

Methods: GC-MS, GLC, methylation analysis, TFA hydrolysis, MALD-MS
Comments, role: N-glycan moiety is composed of a mixture of Hex5-13HexNAc2; absolute D-configurations and pyranose ring sizes were assumed by CSDB staff

Related record ID(s): 43203, 43204
NCBI Taxonomy refs (TaxIDs): 746128
Show glycosyltransferases

There are 4 chemically distinct structures. Please, select:

CC(=O)N[C@@H]1[C@@H](O)[C@H](O[C@@H]2O[C@H](CO)[C@@H](O[C@@H]3O[C@H](CO[C@H]4O[C@H](CO[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5OC5O[C@@H]([C@H](O)CO)[C@H](O)[C@H]5O)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O)[C@@H]4O)[C@@H](O)[C@H](O[C@H]4O[C@H](CO[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H]3O)[C@H](O)[C@H]2NC(C)=O)[C@@H](CO)O[C@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at aDManp: variable unit count (0-6) was assumed to be 0
Warning = sub-repeat ending at ?DGalf: variable unit count (0-1) was assumed to be 0