Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: (Actinopterygii)
Associated disease: vibriosis [ICD11:
XN8RL 
]
The structure was elucidated in this paperNCBI PubMed ID: 9578491Publication DOI: 10.1046/j.1432-1327.1998.2530319.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: eleonora.altman
nrc.ca
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada, Department of Microbiology, College of Biological Sciences, University of Guelph, Canada
Structures of the capsular and O-chain polysaccharides of Vibrio ordalii serotype O:2, the causative agent of vibriosis in salmonid fish, were determined by high-field NMR techniques, mass spectrometric methods and partial hydrolysis. Both polymers were shown to be composed of linear tetrasaccharide repeating units, having the structure: carbohydrate sequence [see text]
Lipopolysaccharide, NMR, capsular polysaccharide, Vibrio ordalii
Structure type: oligomer
Location inside paper: p.324, Table 3
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, composition analysis
Comments, role: the reduced and N-acetylated trisaccharide, obtained by partial hydrolysis of O-PS from V. ordalii serotype O:2.
Related record ID(s): 4395, 4485
NCBI Taxonomy refs (TaxIDs): 28174
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,3,2 Ac
3,4,3 xLAla? ? 50.8 17.7
3,4,2 Ac ? 22.4-23.0
3,4 bDGlcpN3NA 102.7 54.2 55.4 70.7 79.2 ?
3,2 Ac ? 22.4-23.0
3,3 Am ? 19.9
3 bDGlcpN3NA 102.5 53.9 57.5 78.5 78.4 ?
4 Ac ? 22.4-23.0
5 Ac ? 22.4-23.0
xLGal4N5N-onic ? 74.8 79.4 51.9 53.1 61.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,3,2 Ac
3,4,3 xLAla? - 4.23 1.30
3,4,2 Ac - 1.98-2.20
3,4 bDGlcpN3NA 4.61 3.67 3.95 3.64 3.75 -
3,2 Ac - 1.98-2.20
3,3 Am ? 2.19
3 bDGlcpN3NA 4.70 3.89 3.89 3.97 3.80 -
4 Ac - 1.98-2.20
5 Ac - 1.98-2.20
xLGal4N5N-onic - 3.97 4.23 4.27 4.41 3.71
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,3,2 Ac
3,4,3 xLAla? 50.8/4.23 17.7/1.30
3,4,2 Ac 22.4-23.0/1.98-2.20
3,4 bDGlcpN3NA 102.7/4.61 54.2/3.67 55.4/3.95 70.7/3.64 79.2/3.75
3,2 Ac 22.4-23.0/1.98-2.20
3,3 Am ?/? 19.9/2.19
3 bDGlcpN3NA 102.5/4.70 53.9/3.89 57.5/3.89 78.5/3.97 78.4/3.80
4 Ac 22.4-23.0/1.98-2.20
5 Ac 22.4-23.0/1.98-2.20
xLGal4N5N-onic 74.8/3.97 79.4/4.23 51.9/4.27 53.1/4.41 61.1/3.71
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,3,2 | Ac | |
| 3,4,3 | xLAla? |
| 4.23 | 1.30 | |
| 3,4,2 | Ac |
| 1.98
2.20 | |
| 3,4 | bDGlcpN3NA | 4.61 | 3.67 | 3.95 | 3.64 | 3.75 |
|
| 3,2 | Ac |
| 1.98
2.20 | |
| 3,3 | Am | ? | 2.19 | |
| 3 | bDGlcpN3NA | 4.70 | 3.89 | 3.89 | 3.97 | 3.80 |
|
| 4 | Ac |
| 1.98
2.20 | |
| 5 | Ac |
| 1.98
2.20 | |
| | xLGal4N5N-onic |
| 3.97 | 4.23 | 4.27 | 4.41 | 3.71 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,3,2 | Ac | |
| 3,4,3 | xLAla? | ? | 50.8 | 17.7 | |
| 3,4,2 | Ac | ? | 22.4
23.0 | |
| 3,4 | bDGlcpN3NA | 102.7 | 54.2 | 55.4 | 70.7 | 79.2 | ? |
| 3,2 | Ac | ? | 22.4
23.0 | |
| 3,3 | Am | ? | 19.9 | |
| 3 | bDGlcpN3NA | 102.5 | 53.9 | 57.5 | 78.5 | 78.4 | ? |
| 4 | Ac | ? | 22.4
23.0 | |
| 5 | Ac | ? | 22.4
23.0 | |
| | xLGal4N5N-onic | ? | 74.8 | 79.4 | 51.9 | 53.1 | 61.1 |
|
![](nmr_drawer.py?width=750&height=130&nucleus=C&rotate_labels=false&spectrum=[{"s":50.8,"l":"2","c":[0,0,225]},{"s":17.7,"l":"3","c":[0,0,225]},{"s":22.7,"l":"2","c":[0,0,0]},{"s":102.7,"l":"1","c":[230,0,30]},{"s":54.2,"l":"2","c":[230,0,30]},{"s":55.4,"l":"3","c":[230,0,30]},{"s":70.7,"l":"4","c":[230,0,30]},{"s":79.2,"l":"5","c":[230,0,30]},{"s":22.7,"l":"2","c":[0,0,0]},{"s":19.9,"l":"2","c":[0,165,0]},{"s":102.5,"l":"1","c":[120,0,225]},{"s":53.9,"l":"2","c":[120,0,225]},{"s":57.5,"l":"3","c":[120,0,225]},{"s":78.5,"l":"4","c":[120,0,225]},{"s":78.4,"l":"5","c":[120,0,225]},{"s":22.7,"l":"2","c":[0,0,0]},{"s":22.7,"l":"2","c":[0,0,0]},{"s":74.8,"l":"2","c":[0,192,255]},{"s":79.4,"l":"3","c":[0,192,255]},{"s":51.9,"l":"4","c":[0,192,255]},{"s":53.1,"l":"5","c":[0,192,255]},{"s":61.1,"l":"6","c":[0,192,255]}])
The spectrum also has 9 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
Found 
report error