Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 12374825Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: ulrich.dobrindt
mail.uni-wuerzburg.de
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institut fur Molekulare Infektionsbiologie, Bayerische Julius-Maximilians-Universitat Wurzburg, Wurzburg, Germany, Forschungszentrum Borstel, Zentrum fur Medizin und Biowissenschaften, 23845 Borstel, Institut fur Mikrobiologie und Genetik and Gottingen Genomics Laboratory, Georg-August-Universitat Gottingen, 37077 Gottingen, and Bereich Medizin6 and Abteilung Biologische Forschung, Ardeypharm GmbH, 58313 Herdecke, Germany
Structural analysis of lipopolysaccharide (LPS) isolated from semirough, serum-sensitive Escherichia coli strain Nissle 1917 (DSM 6601, serotype O6:K5:H1) revealed that this strain's LPS contains a bisphosphorylated hexaacyl lipid A and a tetradecasaccharide consisting of one E. coli O6 antigen repeating unit attached to the R1-type core. Configuration of the GlcNAc glycosidic linkage between O-antigen oligosaccharide and core (b) differs from that interlinking the repeating units in the E. coli O6 antigen polysaccharide (a). The wa* and wb* gene clusters of strain Nissle 1917, required for LPS core and O6 repeating unit biosyntheses, were subcloned and sequenced. The DNA sequence of the wa* determinant (11.8 kb) shows 97% identity to other R1 core type-specific wa* gene clusters. The DNA sequence of the wb* gene cluster (11 kb) exhibits no homology to known DNA sequences except manC and manB. Comparison of the genetic structures of the wb*O6 (wb* from serotype O6) determinants of strain Nissle 1917 and of smooth and serum-resistant uropathogenic E. coli O6 strain 536 demonstrated that the putative open reading frame encoding the O-antigen polymerase Wzy of strain Nissle 1917 was truncated due to a point mutation. Complementation with a functional wzy copy of E. coli strain 536 confirmed that the semirough phenotype of strain Nissle 1917 is due to the nonfunctional wzy gene. Expression of a functional wzy gene in E. coli strain Nissle 1917 increased its ability to withstand antibacterial defense mechanisms of blood serum. These results underline the importance of LPS for serum resistance or sensitivity of E. coli.
Lipopolysaccharide, antigen, core, gene, O-antigen, Escherichia, Escherichia coli, lipid A, SR-type LPS
Structure type: oligomer
Location inside paper: p.5919, Table 5
Aglycon: lipid A
Compound class: LPS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130648,IEDB_130650,IEDB_130659,IEDB_130670,IEDB_131186,IEDB_133751,IEDB_135813,IEDB_135818,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_140088,IEDB_141584,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_7,SB_72
Methods: GLC-MS, NMR, MALDI-TOF MS
Biological activity: serological study
Biosynthesis and genetic data: genetic data
Comments, role: semirough lipopolysaccharide (serogroup O6)
NCBI Taxonomy refs (TaxIDs): 316435,
217992,
562Reference(s) to other database(s): GTC:G39698ON
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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