Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ];
dysentery [ICD11:
SA56 ];
infection due to Escherichia coli [ICD11:
XN6P4 ]
The structure was elucidated in this paperNCBI PubMed ID: 8864227Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm Unit ersit3, Stockholm, Sweden, Karolinska Institute, Department of Immunology Microbiology, Pathology and Infectious Diseases, Division of Clinical Bacteriology Huddinge University Hospital, S-141 86 Huddinge, Sweden
The structure of the O-specific side-chain of the lipopolysaccharide from Escherichia coli O28 has been investigated. NMR spectroscopy has been the main method used, complemented with sugar and methylation analyses. The polysaccharide contains one equivalent of O-acetyl groups per repeating unit. Selective cleavage of the O-deacetylated polymer was performed by treatment with aqueous hydrofluoric acid, and resulted in a trisaccharide-glycerol. The polysaccharide thus is of the teichoic acid type and composed of repeating units in which the trisaccharide-glycerol residues are joined by phosphodiester linkages. The O-antigen polysaccharide has the following structure. [sequence: see text] The absolute configuration of the glycerol moiety as R, (i.e., D-glycerol 1-phosphate) was determined by a new method based on TEMPO oxidation of the polysaccharide, followed by GLC analysis of the (+)-2-butyl ester of the resulting glyceric acid
Lipopolysaccharide, Escherichia coli, phosphodiester, Teichoic acid type, Enteroinvasive
Structure type: oligomer
Location inside paper: Sceme 1
Contained glycoepitopes: IEDB_135813,IEDB_136095,IEDB_137340,IEDB_137472,IEDB_141807,IEDB_151531,IEDB_190606
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, FAB-MS, sugar analysis, 31P NMR
Comments, role: dephosphorylation product
Related record ID(s): 4385, 4477
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G23359RR
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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